Human CellExp™ PLGF/PIGF2/PGF, human recombinant

• Catalog number
  7238-10
• Price
  Please ask
• Size
  10 ug

• Synonyms
  PGF, PLGF, PlGF2, PlGF, PGFL, SHGC-10760
• Alternative_names
  PGF, PLGF, PlGF2, PlGF, PGFL, SHGC-10760
• Description
  A key molecule in angiogenesis and vasculogenesis
• Recombinant
  Yes
• Source
  HEK 293 cells
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Endotoxin Level
  < 0.1 ng/ug of protein (
• Activity Specifications test method
  Measured in a cell proliferation assay using MDA - MB – 231 human breast cancer cells. The ED50 for this effect is typically 1 - 10 µg/ml.
• Biological activity
  Measured in a cell proliferation assay using MDA - MB – 231 human breast cancer cells. The ED50 for this effect is typically 1 - 10 µg/ml.
• Molecular Weight
  The protein is fused with 6×His tag at the C-terminus, has a calculated MW of 20.2 kDa. The predicted N-terminus is Leu 19. DTT-reduced Protein migrates as 28-33 kDa due to glycosylation.
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized powder
• Physical form description
  Lyophilized from 0.22 um filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
• Reconstitution Instructions
  Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
• Background Information
  Placental growth factor (PGF) also known as vascular endothelial growth factor-related protein, PLGF and PlGF2, is a member of the VEGF (vascular endothelial growth factor) sub-family - a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. The main source of PGF during pregnancy is the placental trophoblast. PGF is also expressed in many other tissues, including the villous trophoblast. PGF is actived in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. PlGF2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CellExp™   PLGF/PIGF2/PGF  
• Gene symbol
  PGF
• Short name
  CellExp™ PLGF/PIGF2/PGF, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Humans
• Alternative name
  H. sapiens CellExp™ PLGF/PIGF2/PGF, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:8893
• Gene
  PGF
• Long gene name
  placental growth factor
• Synonyms gene
  • PGFL
• Synonyms gene name
  • placental growth factor-like
  • placental growth factor, vascular endothelial growth factor-related protein
• Synonyms
  • PlGF-2
  • SHGC-10760
  • D12S1900
  • PIGF
  • PLGF
  • PlGF
• Synonyms name
  • placenta growth factor
• GenBank acession
  • S72960
• Locus
  14q24.3
• Discovery year
  1994-01-14
• Entrez gene record
  5228
• Pubmed identfication
  • 7681160
• RefSeq identity
  • NM_002632
• Classification
  • VEGF family
• VEGA ID
  OTTHUMG00000171496

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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