Human stem cell pluripotent markers (incl. internal control) Multiplex (Oct-4, Sox-2, Rex1, NANOG)
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Catalog number
K235
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Price
Please ask
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Size
100 reactions
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Kind
Conventional PCR-Kits (gel agarose)
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Description
For cells, cell lines and tissues in culture till half confluency. Isotype or positive controls by peptides, antibodies and deactivated samples.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.
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Gene target
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Gene symbol
NANOG
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Short name
stem pluripotent markers (incl. internal control) Multiplex (Oct-4, Sox-2, Rex1, NANOG)
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Technique
multiplex, Control, controls, multiplexes
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Species
Human, Humans
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Alternative name
H. sapiens progenitor cellular pluripotent markers (incl. middlesequence reference) Multiplex (Oct-4, Sox-2, Rex1, NANOG)
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Alternative technique
pcrkits, controls
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Tissue
cell, control, stem
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Gene info
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Identity
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Gene
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Long gene name
Nanog homeobox
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Synonyms
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GenBank acession
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Locus
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Discovery year
2003-09-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- NKL subclass homeoboxes and pseudogenes
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Methods for using more than one primer set in a polymerase chain reaction to amplify more than one segment of the target DNA sequence in a single reaction.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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