Human stem cell endoderm lineage markers (incl. internal control) Multiplex and singlex, Double Check (AFP, Oct-4, BMP-4, HNF 3-β)
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Catalog numberK242
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PricePlease ask
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Size100 reactions
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KindConventional PCR-Kits (gel agarose)
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DescriptionFor cells, cell lines and tissues in culture till half confluency. Isotype or positive controls by peptides, antibodies and deactivated samples.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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TestStem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.
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Gene target
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Gene symbolBMP4, AFP, GDF11, GDF10, ONECUT1, BMP1, GDF2, MIR1302-4, PIRC18
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Short namestem endoderm lineage markers (incl. internal control) Multiplex singlex, Double Check (AFP, Oct-4, BMP-4, HNF 3-β)
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Techniquemultiplex, Control, controls, multiplexes
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SpeciesHumans
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Alternative nameH. sapiens progenitor cellular endoderm lineage markers (incl. middlesequence reference) Multiplex and singlex, Double Check (Alpha fetoprotein, Oct-4, BMP-4, HNF 3-β)
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Alternative techniquepcrkits, controls
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Tissuecell, control, stem
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Viruslineage
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Gene info
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Identity
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Gene
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Long gene namebone morphogenetic protein 4
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Synonyms gene
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GenBank acession
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Locus
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Discovery year1990-06-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Bone morphogenetic proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namealpha fetoprotein
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-04-01
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Entrez gene record
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Pubmed identfication
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namegrowth differentiation factor 11
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-12-02
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Entrez gene record
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Pubmed identfication
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Classification
- Transforming growth factor beta superfamily
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namegrowth differentiation factor 10
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-09-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Transforming growth factor beta superfamily
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameone cut homeobox 1
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Synonyms gene
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Synonyms gene name
- one cut domain, family member 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-02-11
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Entrez gene record
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Pubmed identfication
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Classification
- CUT class homeoboxes and pseudogenes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namebone morphogenetic protein 1
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Synonyms gene
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Synonyms gene name
- procollagen C-endopeptidase
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Synonyms
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Synonyms name
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Locus
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Discovery year1990-06-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Bone morphogenetic proteins
- Astacins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namegrowth differentiation factor 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-09-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Transforming growth factor beta superfamily
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1302-4
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1302 family
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 18
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
MeSH Data
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Name
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ConceptScope note: Methods for using more than one primer set in a polymerase chain reaction to amplify more than one segment of the target DNA sequence in a single reaction.
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Tree numbers
- E05.393.620.500.487
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data