Human stem cell endoderm lineage markers (incl. internal control) Multiplex and singlex, Double Check (AFP, Oct-4, BMP-4, HNF 3-β)

  • Catalog number
    K242
  • Price
    Please ask
  • Size
    100 reactions
  • Kind
    Conventional PCR-Kits (gel agarose)
  • Description
    For cells, cell lines and tissues in culture till half confluency. Isotype or positive controls by peptides, antibodies and deactivated samples.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Test
    Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.
  • Gene target
    stem   endoderm   lineage   markers   incl   internal   singlex   Double   Check   AFP   Oct-4   BMP-4   HNF   3-β  
  • Gene symbol
    BMP4, AFP, GDF11, GDF10, ONECUT1, BMP1, GDF2, MIR1302-4, PIRC18
  • Short name
    stem endoderm lineage markers (incl. internal control) Multiplex singlex, Double Check (AFP, Oct-4, BMP-4, HNF 3-β)
  • Technique
    multiplex, Control, controls, multiplexes
  • Species
    Humans
  • Alternative name
    H. sapiens progenitor cellular endoderm lineage markers (incl. middlesequence reference) Multiplex and singlex, Double Check (Alpha fetoprotein, Oct-4, BMP-4, HNF 3-β)
  • Alternative technique
    pcrkits, controls
  • Tissue
    cell, control, stem
  • Virus
    lineage
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 18
  • Locus
    4
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
MeSH Data
  • Name
  • Concept
    Scope note: Methods for using more than one primer set in a polymerase chain reaction to amplify more than one segment of the target DNA sequence in a single reaction.
  • Tree numbers
    • E05.393.620.500.487
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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