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Available ordering format
Liquid
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Immunogen
Purified rat IgG (H&L)
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified Rabbit IgG
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
2-8°C.
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Verified applications
Contact Gentaur support center
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Connected products
other unconjugated secondary antibodies against rat IgG (H&L)
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Recommended dilutions for use
This antibody is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator.
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
to be determined
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
Refer to PubMed
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Scientific context
Rabbit anti-rat IgG (H&L) is a secondary antibody which binds to rat IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rat IgG (H&L).
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Notes
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rat IgG, light chains on all rat immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rat serum proteins, human serum proteins
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Test
A high affinity purification column was use to purify Rabbit, , Unconjugated, min. cross-reactivity serum by agisera by chromatographic size exclusion.
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Description
1 of protein A or G purified by agisera will give more specificity than crude serum. Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Serum should be stored at +5°C
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Latin name
Oryctolagus cuniculus
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Group
Sera
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Conjugation
not conjugated
