FITC-Linked Polyclonal Antibody to Carcinoembryonic Antigen Related Cell Adhesion Molecule 7 (CEACAM7)
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Catalog number
MBS2069292
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Price
Please ask
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Size
0,1 mg
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Other size
please contact us to order other different size
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Description
cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the FITC-Linked Polyclonal Antibody Carcinoembryonic Antigen Adhesion Molecule 7 (CEACAM7). Antigens are peptides or recombinant or native dependent on the production method. For cells, cell lines and tissues in culture till half confluency. Whole adhesion and interacting molecules are present in lysates used as reference for ELISA quantification of these molecules and their subunits.
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Properties
If you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translation
anticorps
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Gene target
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Gene symbol
CEACAM7
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Short name
FITC-Linked Polyclonal Antibody Carcinoembryonic Antigen Adhesion Molecule 7 (CEACAM7)
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Technique
Polyclonal, Antibody, antigen, FITC, antibodies against human proteins, antibodies for, antigenes, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Label
FITC
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Alternative name
fluorecein-Linked polyclonal (antibody to-) to Carcinoembryonic protein Related cellular Adhesion Molecule 7 (carcinoembryonic antigen-related cellular adhesion molecule 7)
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Alternative technique
polyclonals, antibodies, antigenes, fluorescine
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Alternative to gene target
carcinoembryonic antigen-related cell adhesion molecule 7, CEA and CGM2, CEACAM7 and IDBG-53139 and ENSG00000007306 and 1087, protein binding, Plasma membranes
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Tissue
cell
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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