Carcinoembryonic Antigen Related Cell Adhesion Molecule 1 (CEACAM1) Polyclonal Antibody (Rat), FITC
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Catalog number
PAC977Ra01-200ul-FITC
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Price
Please ask
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Size
200ul
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Description
A Rabbit polyclonal antibody against Rat Carcinoembryonic Antigen Related Cell Adhesion Molecule 1 (CEACAM1). This antibody is labeled with FITC.
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Specifications
Host: Rabbit; Species Reactivity: Rat; Clonality: polyclonal; Tested applications: WB, IHC; Concentration: 500ug/ml; Isotype: IgG; Conjugation: FITC
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Additional_information
Sequence of the immunogen: CEACAM1 (Val36~Gln145); Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
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Storage_and_shipping
Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
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Notes
Research Use Only.
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Additional description
cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the Carcinoembryonic Antigen Adhesion Molecule 1 (CEACAM1) Polyclonal Antibody ( ), FITC. Antigens are peptides or recombinant or native dependent on the production method. For cells, cell lines and tissues in culture till half confluency. Whole adhesion and interacting molecules are present in lysates used as reference for ELISA quantification of these molecules and their subunits.
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Properties
If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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French translation
anticorps
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Gene target
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Gene symbol
CEACAM1
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Short name
Carcinoembryonic Antigen Adhesion Molecule 1 (CEACAM1) Polyclonal Antibody ( ), FITC
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Technique
Polyclonal, Antibody, antigen, FITC, antibodies against human proteins, antibodies for, antigenes, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rat
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Label
FITC
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Species
Rat, Rats
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Alternative name
Carcinoembryonic protein Related cellular Adhesion Molecule 1 (carcinoembryonic antigen-related cellular adhesion molecule 1 (biliary glycoprotein)) polyclonal (antibody to-) (Rat), fluorecein
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Alternative technique
polyclonals, antibodies, antigenes, fluorescine
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Alternative to gene target
carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein), BGP and BGP1 and BGPI, CEACAM1 and IDBG-54381 and ENSG00000079385 and 634, protein homodimerization activity, Plasma membranes
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Tissue
cell
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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