Mouse Anti-Mouse Protocadherin Gamma B2 Monoclonal IgG1, Clone S148-30
-
Catalog numberSMC-452D-ALP
-
PricePlease ask
-
Size100 µg
-
-
CloneS148-30
-
ImmunogenFusion protein amino acids 719-804 (variable cytoplasmic domain) of mouse Gamma- Protocadherin Gamma B2. Human: 68% identity (60/88 amino acids identical) >60% identity with other Gamma-protocadherin-B proteins
-
Antibody s full descriptionMouse Anti-Mouse Protocadherin Gamma B2 Monoclonal IgG1 Antibody, Clone: S148-30: Alkaline Phosphatase
-
Antibody s categoryMonoclonal Antibodies
-
Antibody s other namePCDH-gamma-B2 Antibody, PCDHGB2 Antibody, Protocadherin gamma B2 Antibody, Protocadherin gamma subfamily B Antibody, 2 Antibody, Protocadherin gamma subfamily B2 Antibody, Gamma Protocadherin-B2 Antibody, Gamma Protocadherin B2 Antibody
-
Raised inMouse
-
Antibody s targetProtocadherin Gamma B2
-
Primary research fieldsNeuroscience, Cell Structure, Membrane Markers
-
BrandnameNone
-
Antibodies applicationsWB, ICC/IF
-
Antibody s reactivityHuman, Mouse
-
Antibody s dilutionsWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
-
PurityProtein G Purified
-
Antibody buffer for storagePBS pH 7.4, 50% glycerol, 0.1% sodium azide
-
Antibody s concentration1 mg/ml
-
Antibody s specificityDetects ~100kDa. No cross-reactivity against other Protocadherin Gamma -A, -B or -C proteins.
-
Storage recommendations-20ºC
-
Shipping recommendationsBlue Ice or 4ºC
-
Antibody certificate of analysis1 µg/ml of SMC-452 was sufficient for detection of Protocadherin Gamma B2 in 20 µg of COS cell lysate (transiently transfected with GFP-tagged Protocadherin Gamma B2) by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
-
Antibody in cellCell Membrane
-
Tissue specificitySee included datasheet or contact our support service
-
Scientific contextProtocadherins are a large family of cadherin-like cell adhesion proteins that are involved in the establishment and maintenance of neuronal connections in the brain. There are three protocadherin (PCDH) gene clusters, designated alpha, beta and gamma, all of which contain multiple tandemly arranged genes. PCDHGA3 (Protocadherin gamma-A3) is a 932 amino acid that is one of 22 proteins encoded by the protocadherin gamma cluster. The protocadherein gamma cluster consists of three subfamilies (A, B and C) and PCDHGA3 is a member of the gamma subfamily A. PCDHGA3 is a type I transmembrane receptor containing six cadherin motifs and is expressed in the central nervous system where it localizes to synapses. Members of the gamma cluster of protocadherins are essential for neuronal survival. There are two isoforms of PCDHGA3 that are produced as a result of alternative splicing events.
-
Released date20-Nov-2013
-
Tested applicationsTo be tested
-
Tested reactivityTo be tested
-
NCBI numberNP_291053.1
-
Gene number93700
-
Protein numberQ91XX7
-
Antibody s datasheetContact our support service
-
Representative figure link
-
Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Protocadherin Gamma B2 Monoclonal Antibody, Clone S148-30 (SMC-452). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Protocadherin Gamma B2 Monoclonal Antibody (SMC-452) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoskeleton. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Protocadherin Gamma B2 Antibody (D) Composite. Western Blot analysis of Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-B2 showing detection of ~100 kDa Protocadherin Gamma B2 protein using Mouse Anti-Protocadherin Gamma B2 Monoclonal Antibody, Clone S148-30 (SMC-452). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-B2. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Protocadherin Gamma B2 Monoclonal Antibody (SMC-452) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~100 kDa. Mouse Anti-Protocadherin Gamma B2 Antibody [S148-30] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-452) Mouse Anti-Protocadherin Gamma B2 Antibody [S148-30] used in Western Blot (WB) on Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-B2 (SMC-452)
-
Warning informationNon-hazardous
-
Country of productionCanada
-
Total weight kg1.4
-
Net weight g0.1
-
Stock availabilityIn Stock
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin nameMus musculus
-
Gene target
-
Gene symbolSNORD114-30, SNORD116-30, SNORD115-30, IGKV2-30, RNU6-30P, IGHV3-30, IGLV3-30, IGHVII-30-21, IGHVII-30-1, IGKV2D-30
-
Short nameMouse Anti-Mouse Protocadherin Gamma B2 Monoclonal IgG1, Clone S148-30
-
Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
-
Hostmouse
-
IsotypeIgG1, IgG1
-
LabelAlkaline Phosphatase
-
SpeciesMouse, Mouses
-
Alternative nameMouse Antibody toMouse Protocadherin g B2 monoclonal IgG1, clonality S148-30
-
Alternative techniquemurine, antibodies
-
Clone nameS148-30
-
Gene info
-
Identity
-
Gene
-
Long gene namesmall nucleolar RNA, C/D box 114-30
-
Synonyms
-
Locus
-
Discovery year2006-09-26
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small nucleolar RNAs, C/D box
Gene info
-
Identity
-
Gene
-
Long gene namesmall nucleolar RNA, C/D box 116-30
-
Synonyms
-
Locus
-
Discovery year2011-08-22
-
Entrez gene record
-
RefSeq identity
-
Classification
- Small nucleolar RNAs, C/D box
Gene info
-
Identity
-
Gene
-
Long gene namesmall nucleolar RNA, C/D box 115-30
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2007-02-15
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small nucleolar RNAs, C/D box
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin kappa variable 2-30
-
GenBank acession
-
Locus
-
Discovery year2000-04-18
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin kappa locus at 2p11.2
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameRNA, U6 small nuclear 30, pseudogene
-
Synonyms gene
-
Synonyms gene name
- RNA, U6 small nuclear 30
-
Locus
-
Discovery year2008-06-10
-
Entrez gene record
-
RefSeq identity
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin heavy variable 3-30
-
GenBank acession
-
Locus
-
Discovery year2000-04-04
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin heavy locus at 14q32.33
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin lambda variable 3-30 (pseudogene)
-
Synonyms gene name
- immunoglobulin lambda variable 3-30
- immunoglobulin lambda variable 3-30 pseudogene
-
GenBank acession
-
Locus
-
Discovery year2000-05-08
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin lambda locus at 22q11.2
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin heavy variable (II)-30-21 (pseudogene)
-
Synonyms
-
Locus
-
Discovery year2016-11-10
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin heavy locus at 14q32.33
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin heavy variable (II)-30-1 (pseudogene)
-
Synonyms gene name
- immunoglobulin heavy variable (II)-30-1
- immunoglobulin heavy variable (II)-30-1 pseudogene
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2000-04-17
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin heavy locus at 14q32.33
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin kappa variable 2D-30
-
GenBank acession
-
Locus
-
Discovery year2000-04-18
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin kappa locus at 2p11.2
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data