ApoBrdU Red DNA Fragmentation Kit
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Catalog number
K404-60
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Price
Please ask
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Size
60 assays
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Description
Detects DNA Fragmentation by FACS or FL
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Summary
• Detection method- Flow cytometry (Ex/Em = 488/576 nm for BrdU-Red and 488/655 nm for 7-AAD). • Species reactivity- Mammalian • Applications- The TUNEL-based immunohistochemical staining kit provides complete reagents including positive and negative control cells for convenient detection of DNA fragmentation
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Sample Type
Tissue sections and fixed cell preparations
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Features Benefits
• Simple procedure; takes ~ 3 hours • Fast and convenient • The assay is sensitive and stable • The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. BioVision’s Apo-BrdU-Red In Situ DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more readily incorporated into DNA strand breaks than other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater incorporation gives rise to brighter signal when the Br-dUTP sites are identified by a Red fluorescence labeled anti-BrdU monoclonal antibody. The assay is suitable for studying apoptosis with GFP transfected cells.
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Conjugation
red dye
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Gene target
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Gene symbol
PRKDC, HLA-DOA
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Short name
ApoBrdU Red DNA Fragmentation Kit
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Technique
dna
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Label
Red
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Alternative name
ApoBrdU Red Desoxyribonucleic acid Fragmentation reagent
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Alternative technique
kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
Gene info
Product images
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