- Catalog numberK401-60
- Product nameApoBrdU DNA Fragmentation Assay Kit
- Size60 assays
- PriceAsk For Price
- DescriptionDetects DNA Fragmentation by FACS or FL
- Summary• Detection method- Flow cytometry (Ex/Em = 488/520 nm for FITC, and 488/623 nm for PI) and fluorescence microscopy (FITC and rhodamine filters) • Species reactivity- Mammalian • Applications- The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.
- Sample TypeCell cultures (adherent and suspension), parafin embedded tissue sections
- Features Benefits• Simple procedure; takes ~ 1 hour • Fast and convenient • The assay is sensitive and stable • The incorporation of BrdU in the procedure provides greater sensitivity than biotinylated or digoxigenylated methods.
- Storage Conditions-20°C
- Shipping Conditionsgel pack
- Shelf life12 months
- BackgroundInternucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. BioVision’s Apo-BrdU In Situ DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more readily incorporated into DNA strand breaks than other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater incorporation gives rise to brighter signal when the Br-dUTP sites are identified by a fluorescein labeled anti-BrdU monoclonal antibody.
- Data Sheet Link
- Alternative to gene target
- [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
- Gene targetApoBrdU DNA Fragmentation Kit
- Identity:HGNC:9413
- Gene:PRKDC
- Long gene name:protein kinase, DNA-activated, catalytic subunit
- Synonyms gene name:protein kinase, DNA-activated, catalytic polypeptide
- Synonyms:DNPK1p350DNAPKXRCC7DNA-PKcsDNAPKcDNA-PKCp460
- Discovery year:1993-11-09
- Entrez gene record:5591
- Pubmed identification:7638222
- RefSeq identity:NM_001081640
- Classification:Armadillo like helical domain containing
- VEGA ID:OTTHUMG00000164239
- Identity:HGNC:4936
- Gene:HLA-DOA
- Long gene name:major histocompatibility complex, class II, DO alpha
- Synonyms:HLA-D0-alpha
- Discovery year:2001-06-22
- Entrez gene record:3111
- Pubmed identification:2370084
- RefSeq identity:NM_002119
- Classification:C1-set domain containing, Histocompatibility complex
- VEGA ID:OTTHUMG00000031211
- Gene symbolPRKDC, HLA-DOA
- HostAssay
- Short nameApoBrdU DNA Fragmentation Assay Kit
- technique filter
- Assay
- dna
- TechniqueAssay, dna, assays
- Alternative techniquearrays, kits
Gene info
Locus Specific Databases:LRG_162
Gene info
Locus Specific Databases:IMGT/HLA Database