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Available ordering format
Inquire
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Immunogen
KLH-conjugated synthetic peptide derived from all known plant,algal and cyanobacterial RbcL (Rubisco large subunit of Rubisco Form I) sequences, including Arabidopsis thaliana UniProt: O03042, TAIR: AtCg00490, Synechococcus sp. Q3ALL1
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Raised in
Hen
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Total IgY in PBS pH 8.0+ 0.02% sodium azide
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), immunolocalization (IL)
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Connected products
AS03 037 | anti-RbcL | Rubisco large subunit, form I and form II (50 µl)AS03 037A | anti-RbcL | Rubisco large subunit, form I and form II (50 µg affinity purified)AS03 037-HRP| anti-RbcL | Rubisco large subunit, form I and form II (40 µg, HRP-conjugated) AS15 2955 | anti-RbcL II | Rubisco large subunit, form II (50 µl), rabbit antibodyAS15 2955S | RbcL II | Rubisco form II positive control/quantitation standardAS01 017S | Rubisco protein standard for quantitative western blot or positive controlAS03 037PRE | Rubisco large subunit, pre-immune serumAS09 409 | Rubisco quantitation kit AS15 2994 | Rubisco ELISA quantitation kit AS07 218 | anti-Rubisco | 557 kDa hexadecamer, rabbit antibody to a whole protein AS07 259 | anti-RbcS | Rubisco small subunit (SSU), rabbit antibodyAS07 222 | anti-RbcS | Rubisco small subunit (SSU) from pea, rabbit antibody Recommended secondary antibodies: Goat anti-chicken IgY (H&L), HRPconjugated or Goat anti-chicken IgY (H&L), ALP conjugatedPlant and algal protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1: 10 000 - 1: 20 000 on 2 µg of total cellular protein, detected with standard ECL (WB); (IL) tested on a grass species, formaldehyde-fixed and paraffin-embedded tissue following the protocol from Gonzalez et al. (1998) Plant Physiol. V. 116.
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Molecular weight expected аpparent
52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 kDa (Chlamydomonas reinhardtii)
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Verified reactivity
Arabidopsis thaliana, Lobaria pulmonaria, Medicago sativa, mixed phytoplankton, Pisum sativum, Solanum tuberosum, Spartina alterniflora, Spinacia oleracea, Synechococcus sp. PCC7842, Thiobacillus sp. Ulmus sp.
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Possible reactivity
di and monocots, green algae, mosses, conifers, liverworts, welwitschia, prochlorophytes
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
This antibody detects RbcL protein from 102.6 fmoles and has been used as a control to ensure adequate permeabilization and fixation of toxic cyanobacterial cells in immunolabeling experiments (method based on: Orellana & Perry (1995) J Phycol 31: 785-794).Antibody has been used in immunolabelling of intact cyanobacterial cells fixed with ethanol using a secondary anti-IgY antibody conjugated with a fluorochrome.
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References
Robert et al. (2015). Leaf proteome rebalancing in Nicotiana benthamiana for upstream enrichment of a transiently expressed recombinant protein. Plant Biotechnol J. 2015 Aug 19. doi: 10.1111/pbi.12452. Morash et al. (2007). Macromolecular dynamics of the photosynthetic system over a seasonal developmental progression in Spartina alterniflora. Canadian J. of Botany, 2007, 85(5): 476-483, 10.1139/B07-043. MacKenzie et al. (2005). Inorganic carbon acclimation in Synechococcus elongatus alters the dynamics of macromolecular pooks and photosynthetic fluxes in response to increased light. Photosynt Research 85: 341-357.Schofield et al. (2003). Changes in macromolecular allocation in nondividins algal symbionts allow for photosynthetic acclimation in the lichen Lobaria pulmonaria. New Phytol 159: 709-718.
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Scientific context
Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthesis. It is one of the most abundant proteins on Earth and its homology has been demonstrated from purple bacteria to flowering plants.
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Notes
Peptide target used to elicit this antibody is not conserved in type II Rubisco found in dinoflagellates and some photosynthetic bacteria. For those species using product AS03 037 is recommended.
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Protein number
O03042 , Q3ALL1
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TAIR number
ATCG00490
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