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Available ordering format
Liquid in PBS pH 7.4, conjugated to HRP
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Immunogen
KLH-conjugated synthetic peptide conserved across all known plant, algal and (cyano)bacterial RbcL protein sequences (form I L8S8 and form II L2), including Arabidopsis thaliana AtCg00490, Hordeum vulgare P05698, Oryza sativa P0C510, Chlamydomonas reinhardtii P00877, Synechococcus PCC 7920 A5CKC5
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store at +4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
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Verified applications
ELISA (ELISA), western blot (WB)
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Connected products
AS03 037 | anti-RbcL | Rubisco large subunit, form I and form II (50 µl)AS03 037A | anti-RbcL | Rubisco large subunit, form I and form II (50 µg affinity purified)AS15 2955 | anti-RbcL II | Rubisco large subunit, form II (50 µl), rabbit antibodyAS15 2955S | RbcL II | Rubisco form II positive control/quantitation standardAS01 017 | anti-RbcL | Rubisco large subunit, form I, chicken antibodyAS01 017S | Rubisco protein standard for quantitative western blot or positive controlAS03 037PRE | Rubisco large subunit, pre-immune serumAS09 409 | Rubisco quantitation kit AS15 2994 | Rubisco ELISA quantitation kit AS07 218 | anti-Rubisco | 557 kDa hexadecamer, rabbit antibody to a whole protein AS07 259 | anti-RbcS | Rubisco small subunit (SSU), rabbit antibodyAS07 222 | anti-RbcS | Rubisco small subunit (SSU) from pea, rabbit antibody matching secondary antibodyPlant and algal protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1: 5000 (ELISA), 1: 5000 - 10 000 with standard ECL (WB)
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Molecular weight expected аpparent
52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 (Chlamydomonas reinhardtii)
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Verified reactivity
Arabidopsis thaliana, Apium graveolens, Artemisia annua, Baculogypsina sphaerulata (benthic foraminifer), Bienertia sinuspersici, Cicer arietinum, Chlamydomonas raudensis, Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Cyanophora paradoxa, Cylindrospermopsis raciborskii CS-505, Emiliana huxleyi, Euglena gracilis, Fraxinus mandshurica, Fucus vesiculosus, Glycine max, Gonyaulax polyedra, Guzmania hybrid, Heterosigma akashiwo, Karenia brevis (C.C.Davis) s) G.Hansen & Ø.Moestrup (Wilson isolate), Liquidambar formosana, Micromonas pusila, Nicotiana benthamiana, Physcomitrella patens, Porphyra sp. , Schima superba, Stanleya pinnata, Spinacia oleracea, lichens, Symbiodinium sp., Synechococcus PCC 7942, Thalassiosira pseudonana, Thermosynechococcus elongatus, Prochlorococcus sp. (surface and deep water ecotype), Triticum aestivum, dinoflagellate endosymbionts (genus Symbiodinium), extreme acidophilic verrucomicrobial methanotroph Methylacidiphilum fumariolicum strain SolV, Thalassiosira punctigera, Vitis vinifera
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Possible reactivity
di and monocots, conifers, mosses, liverworts, welwitschia, green algae, red alge, brown algae, cryptomonad, cyanobacteria including prochlorophytes, gamma-proeobacteria, beta-proteobacteria, alpha proteobacteria, Suaeda glaucaPredicted but not confirmed reactivity for Rubisco form II.
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
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References
Refer to PubMed
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Scientific context
Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.
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Notes
anti-RbcL can be used as a cellular [compartment marker] of plastid stroma (cytoplasm in cyanobacteria) and detects RbcL protein from 31.25 fmoles. As both forms (I and II) are detected it is suitable for work with samples from Dinoflagellates, Haptophytes and Ochrophytes (diatoms, Raphidophytes, brown algae) as well as higher plants. This antibody together with Agrisera Rubisco protein standard is very suitable to quantify Rubisco in plant and algal samples.
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Protein number
O03042 , P05698 , P0C510 , P00877
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TAIR number
ATCG00490
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Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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Conjugation
HRP Conjugated