Active Human Caspases Set IV

• Catalog number
  K233-10-25
• Price
  Please ask
• Size
  10 x 25 units. Contains active caspase-1/-2/-3/-4/-5/-6/-7/-8/-9/-10.

• Synonyms
  Active Human Caspases Set IV
• Alternative_names
  N/A
• Description
  Set of recombinant proteases involved in apoptosis
• Recombinant
  Yes
• Source
  E. coli
• Assay
  SDS-PAGE
• Unit Definition
  One unit of the recombinant human caspases is the enzyme activity that cleaves 1 nmol of the individual caspase substrate (YVAD-pNA for caspase-1, VDVAD-pNA for caspase-2, DEVD-pNA for caspase-3, WEHD-pNA for caspase-4 and –5, VEID-pNA for caspase-6, DEVD-pNA for caspase-7, IETD-pNA for caspase-8 and –10 and LEHD-pNA for caspase-9) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
• Storage Temp
  -70°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Lyophilized powder
• Reconstitution Instructions
  The active recombinant caspases can be reconstituted to 0.1-1 unit per µl in PBS or the Reaction Buffer described above (available from BioVision, Cat.# 1068-20) for longer stability. We recommend using 1 unit per assay for analyzing caspase activity.
• Background Information
  Active recombinant caspases are useful in screening caspase inhibitors, studying enzyme kinetics, determining substrate specificity, and as a positive control in caspase activity assays.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

• Gene target
  Active   Caspases   Set  
• Short name
  Active Caspases Set IV
• Species
  Human, Humans
• Alternative name
  functionnal H. sapiens Caspases Set IV
• Tissue
  set

MeSH Data

• Name
  Models, Chemical
• Concept
  Scope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
• Tree numbers
  • E05.599.495

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