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Synonyms
Active Human Caspases Group I, Active Human
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Alternative_names
N/A
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Description
Set of recombinant proteases involved in apoptosis
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Recombinant
Yes
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Source
E. coli
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Assay
SDS-PAGE
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Unit Definition
One unit of the active recombinant caspase-1,-4,-5 is the enzyme activity that cleaves 1 nmol of the caspase substrate YVAD-pNA (for caspase-1) and WEHD-pNA (for caspase-4 & -5), respectively, per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
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Storage Temp
-70°C
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Shipping
gel pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized powder
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Reconstitution Instructions
Reconstitute to 1 unit/µl in PBS containing 15% glycerol.
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Background Information
The caspase family consists of more than 10 members. Based on their extended substrate specificities, these enzymes were divided into three major groups: Group I (contains caspase-1,-4,-5), Group II (contains caspase-2,-3,-7), and Group III (contains caspase-6,-8,-9,-10). Evidences have suggested that Group I caspases are involved primarily in the production of inflammatory cytokines, while group II and III enzymes function in apoptosis as effectors and upstream activators, respectively. The group I caspases were expressed in E. coli and routinely tested for their ability to enzymatically cleave the substrate YVAD-pNA (for caspase-1) and WEHD-pNA (for caspase-4 & -5).
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
