Active Human Caspases Set II
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Synonyms
Active Human Caspases Set II
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Alternative_names
N/A
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Description
Set of recombinant proteases involved in apoptosis
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Recombinant
Yes
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Source
E. coli
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Assay
SDS-PAGE
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Unit Definition
One unit of the recombinant human caspase-1, -2, -9, and -10 is the enzyme activity that cleaves 1 nmol of the caspase substrate YVAD-pNA, VDVAD-pNA, LEHD-pNA, and IETD-pNA, respectively, per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
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Storage Temp
-70°C
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Shipping
gel pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized powder
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Reconstitution Instructions
Reconstituted to 0.1-1 unit per µl in PBS.
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Background Information
Caspases are known to play an important role in apoptosis. Caspases exist in cells as inactive pro-enzymes. Most of the pro-caspases are matured by proteolysis to yield large and small subunits. Active caspases are heterotetramers, each of which consists of two large and two small subunits.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
MeSH Data
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Name
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Concept
Scope note:
Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
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