Leptin (LEP) Polyclonal Antibody (Bovine), Cy3
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Catalog number
PAA084Bo01-20ul-Cy3
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Price
Please ask
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Size
20ul
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Description
A Rabbit polyclonal antibody against Bovine Leptin (LEP). This antibody is labeled with Cy3.
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Specifications
Host: Rabbit; Species Reactivity: Bovine; Clonality: polyclonal; Tested applications: WB; Concentration: 500ug/ml; Isotype: IgG; Conjugation: Cy3
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Additional_information
Sequence of the immunogen: LEP (Asp29~Gln160); Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
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Storage_and_shipping
Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
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Notes
Research Use Only.
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Properties
If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Leptin (LEP) Polyclonal Antibody ( ), can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Cloud Clone Corp antibody is well suited for fluorescent microscopy.
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Conjugation
cy3 conjugation kit
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Gene
Human or mouse Leptin (from Greek λεπτός leptos, "thin") the "satiety hormone", is a hormone made by adipose cells that helps to regulate energy balance by inhibiting hunger. Leptin is opposed by the actions of the hormone ghrelin, the "hunger hormone". Both hormones act on receptors in the arcuate nucleus of the hypothalamus to regulate appetite to achieve energy homeostasis. ELISA kits and peptides and antibodies are available.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translation
anticorps
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Gene target
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Gene symbol
LEP
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Short name
Leptin (LEP) Polyclonal Antibody ( ),
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Technique
Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Label
Cy3
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Species
Bovine, Bos Taurus genes are available form the Bovine Genome database.
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Alternative name
Leptin (leptin) polyclonal (antibody to-) (Bovine), cyanine 3
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Alternative technique
polyclonals, antibodies
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Alternative to gene target
leptin, LEPD and OB and OBS, LEP and IDBG-39587 and ENSG00000174697 and 3952, peptide hormone receptor binding, Extracellular, Lep and IDBG-130781 and ENSMUSG00000059201 and 16846, OBESE and IDBG-635003 and ENSBTAG00000014911 and 280836
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Gene info
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Identity
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Gene
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Long gene name
leptin
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Synonyms gene
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Synonyms gene name
- leptin (murine obesity homolog)
- leptin (obesity homolog, mouse)
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Locus
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Discovery year
1993-01-26
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Entrez gene record
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Pubmed identfication
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Classification
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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