Cyclin E1 Antibody
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Catalog number6932-50
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PricePlease ask
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Size50 µl
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Antibody TargetCyclin E1
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Target Alternative NameCCNE1, Cyclin Es, Cyclin Et, G1/S-specific cyclin-E1
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse, rat, bovine, dog, pig
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Immunogen SequenceThis Cyclin E1 antibody is generated from KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cyclin E1. The exact sequence is proprietary.
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AccessionP24864
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Gene Id898
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AppearanceColorless liquid
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FormLiquid
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Concentration1 mg/ml
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Formulation1 mg/ml in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
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PurificationImmunogen Affinity chromatography
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ApplicationWB, IHC, IF/IC
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Positive ControlWB: HEK293T, mouse brain, PC12, SW626, HeLa, K562, whole cell lysates, IHC: human lung cancer, IC/IF: PC12 cells
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Application And UsagesWB: 1:500 - 1:1000, IHC: 1:100 – 1:200, IF/IC: 1:100 – 1:500
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DescriptionCyclin E1 is a regulatory subunit of the CDK2 kinase. It is central for the regulation of the G1/S transition and its abundance is tightly regulated throughout the cell cycle via ubiquitination. Cyclin E has been the subject of intense study in relation to tumorigenesis and cancer management and prognosis. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedVerified
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Immunofluorescence VerifiedVerified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Dot Blot VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolCCNE1
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Short nameCyclin E1 Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameCyclin E1 (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namecyclin E1
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Synonyms gene
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Synonyms name
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GenBank acession
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Locus
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Discovery year1991-12-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Cyclins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data