Phosphocreatine (PCr) Polyclonal Antibody (Pan-species), FITC

• Catalog number
  PAV808Ge01-20ul-FITC
• Price
  Please ask
• Size
  20ul

• Description
  A Rabbit polyclonal antibody against Pan-species Phosphocreatine (PCr). This antibody is labeled with FITC.
• Specifications
  Host: Rabbit; Species Reactivity: Pan-species; Clonality: polyclonal; Tested applications: WB; IHC; ICC; IP.; Concentration: 500ug/ml; Isotype: IgG; Conjugation: FITC
• Additional_information
  Sequence of the immunogen: Inquire for antigen sequence.; Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Storage_and_shipping
  Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
• Notes
  Research Use Only.
• Properties
  If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes. Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
• Conjugation
  Anti-FITC Antibody
• Group
  PCR, polymerase chain reaction, Polyclonals and antibodies
• About
  TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• Additional description
  Monoclonals are usually produced in mouse as host species and polyclonals in rabbit or goat. Multi species reactivity is achieved by selecting consensus epitopes when blasting the gene for a reactive, innugenic epitope that is the same in the different target species.
• French translation
  anticorps

• Gene target
  Phosphocreatine   Pan  
• Short name
  Phosphocreatine (PCr) Polyclonal Antibody (Pan- ), FITC
• Technique
  Polyclonal, Antibody, FITC, PCR, antibodies against human proteins, antibodies for, Fluorescein, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Label
  FITC
• Alternative name
  Phosphocreatine (PCR test kit) polyclonal (antibody to-) (Pan-species), fluorecein
• Alternative technique
  polyclonals, antibodies, fluorescine, dna-amplification

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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