Phosphocreatine (PCr) Polyclonal Antibody (Pan-species), HRP
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Catalog number
PAV808Ge01-200ul-HRP
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Price
Please ask
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Size
200ul
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Description
A Rabbit polyclonal antibody against Pan-species Phosphocreatine (PCr). This antibody is labeled with HRP.
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Specifications
Host: Rabbit; Species Reactivity: Pan-species; Clonality: polyclonal; Tested applications: WB; IHC; ICC; IP.; Concentration: 500ug/ml; Isotype: IgG; Conjugation: HRP
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Additional_information
Sequence of the immunogen: Inquire for antigen sequence.; Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
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Storage_and_shipping
Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
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Notes
Research Use Only.
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Properties
If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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Conjugation
HRP Conjugated
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Group
PCR, polymerase chain reaction, Polyclonals and antibodies
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About
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Additional description
Monoclonals are usually produced in mouse as host species and polyclonals in rabbit or goat. Multi species reactivity is achieved by selecting consensus epitopes when blasting the gene for a reactive, innugenic epitope that is the same in the different target species.
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French translation
anticorps
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Gene target
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Short name
Phosphocreatine (PCr) Polyclonal Antibody (Pan- ),
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Technique
Polyclonal, Antibody, PCR, antibodies against human proteins, antibodies for, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Label
HRP
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Alternative name
Phosphocreatine (PCR test kit) polyclonal (antibody to-) (Pan-species), horseradish peroxidase
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Alternative technique
polyclonals, antibodies, dna-amplification
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MeSH Data
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Name
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Concept
Scope note:
Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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