-
Synonyms
Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1, AAT, PI
-
Alternative_names
Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1, AAT, PI
-
Description
An acute-phase plasma protein
-
Recombinant
No
-
Source
Human Plasma
-
Purity by SDS PAGE
≥95%
-
Assay
SDS-PAGE
-
Biological activity
Shows 75-100% inhibition when tested with active-site titrated porcine pancreatic trypsin using Na-Benzoyl-L-Arginine-para-Nitroanilide Hydrochloride (L-BAPNA) as substrate.
-
Results
≥ 5.0 IU/mg
-
Molecular Weight
52 kDa
-
Storage Temp
-20°C
-
Shipping
Gel Pack
-
Shelf Life
12 months
-
Appearance
Lyophilized
-
Physical form description
Lyophilized from 30 mM Na Phosphate, pH 6.5 with 300 mM NaCl.
-
Reconstitution Instructions
In water or aqueous buffer
-
Background Information
Alpha 1 Antitrypsin is an acute-phase plasma protein found at 290 mg per 100 ml. It functions as a serine protease inhibitor with targets like elastase, plasmin, collagenase, thrombin, leucocyte proteases, trypsin, chymotrypsin, and plasminogen activator. Clinically, its deficiency is associated with two major diseases: pulmonary emphysema and early onset/juvenile hepatic cirrhosis. It is elevated in inflammatory conditions, malignancies, liver disease and pregnancy and also after surgical trauma and use of oral contraceptives. The simultaneous quantitative determination of alpha-1-PI and ceruloplasmin permits differential diagnosis of liver afflictions. Rise in alpha 1 antitrypsin occurs as an acute phase response to tissue necrosis and inflammation.
-
Handling
Centrifuge the vial prior to opening.
-
Usage
For Research Use Only! Not to be used in humans
-
-
Additional description
The Alpha 1 Antitrypsin, Plasma is a α- or alpha protein sometimes glycoprotein present in blood.
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
