0.5mg/ml if reconstituted with 0.2ml sterile DI water
Antigen affinity purified
Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
Western blot: 0.5-1ug/ml
The stated application concentrations are suggested starting amounts. Titration of the ABCG1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
ABCG1 (ATP-binding cassette, subfamily G, member 1) also known as ABC8, is a protein that in humans is encoded by the ABCG1 gene. ABCG1 is a regulator of macrophage cholesterol and phospholipid transport. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. The gene is mapped on 21q22.3. This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types. ABCG1 is induced in monocyte-derived macrophages during cholesterol influx mediated by acetylated low-density lipoprotein. Inhibition of ABCG1 protein expression using an antisense strategy resulted in reduced HDL3-dependent efflux of cholesterol and choline-phospholipids. In macrophages, silencing of GPS2 by RNA interference reducedABCG1expression and diminished ABCG1-mediated cholesterol efflux.
An amino acid sequence from the C-terminus of human ABCG1 (ETCHFQKSEAILRELDVENAK) was used as the immunogen for this ABCG1 antibody (100% homologous in human, mouse and rat).
After reconstitution, the ABCG1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.