SIGLEC7 Antibody

• Catalog number
  F40103-0.4ML
• Price
  Please ask
• Size
  0.4 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  SIGLEC7
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC, ELISA
• Recommended dilutions
  Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
• Notes
  Titration of the SIGLEC7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This SIGLEC7 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q9Y286
• Purity
  Antigen affinity
• Description
  SIGLECs are cell surface proteins of the Ig superfamily. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, or ITIMs. A large subgroup of SIGLECs share high homology with SIGLEC3 (CD33) and are localized to 19q13.4. The cDNA for the SLG gene encodes 2 variants, SLG-long (SLGL) and SLG-short (SLGS). The 595-amino acid SLGL protein contains a signal peptide and 2 V-set N-terminal Ig-like domains. The 477-amino acid SLGS protein has a weak signal sequence and, like most SIGLEC3-like SIGLECs, has only 1 V-set N-terminal Ig-like domain. Both variants contain 2 C2-set N-terminal Ig-like domains, a transmembrane domain, and a cytoplasmic tail with a putative ITIM and a putative SLAM-like tyrosine-based motif. The conserved arginine residue thought to be essential for sialic acid binding in other SIGLECs is replaced by a glutamine in SLGS and by a cysteine in SLGL. RT-PCR analysis detected high expression of both variants in spleen and small intestine, and SLGS was highly expressed in adrenal gland and SLGL was highly expressed in bone marrow.
• Immunogen
  A portion of amino acids 1-30 from the human protein was used as the immunogen for this SIGLEC7 antibody.
• Storage
  Aliquot the SIGLEC7 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  SIGLEC7  
• Gene symbol
  SIGLEC7
• Short name
  Anti-SIGLEC7
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit Ig
• Alternative name
  Antibodies to SIGLEC7
• Alternative technique
  antibodies
• Alternative to gene target
  sialic acid binding Ig-like lectin 7, SIGLEC7 and IDBG-65680 and ENSG00000168995 and 27036, carbohydrate binding, Plasma membranes

Gene info

• Identity
  HGNC:10876
• Gene
  SIGLEC7
• Long gene name
  sialic acid binding Ig like lectin 7
• Synonyms gene
  • SIGLEC19P
  • SIGLECP2
• Synonyms gene name
  • sialic acid binding Ig-like lectin 19, pseudogene
  • sialic acid binding Ig-like lectin, pseudogene 2
  • sialic acid binding Ig-like lectin 7
• Synonyms
  • SIGLEC-7
  • p75/AIRM1
  • QA79
  • CD328
• GenBank acession
  • AF170485
• Locus
  19q13.41
• Discovery year
  2000-01-26
• Entrez gene record
  27036
• Pubmed identfication
  • 10567377
• RefSeq identity
  • NM_016543
• Classification
  • CD molecules
  • Sialic acid binding Ig like lectins
  • V-set domain containing
• VEGA ID
  OTTHUMG00000182895

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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