Mouse Anti-Mouse LRP4 (Cytoplasmic) Monoclonal IgG1, Clone S164-6
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Catalog numberSMC-415D-FITC
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PricePlease ask
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Size100 µg
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CloneS164-6
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ImmunogenFusion protein amino acids 1747-1905 (cytoplasmic C-terminus) of mouse LRP4
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Antibody s full descriptionMouse Anti-Mouse LRP4 Monoclonal IgG1 Antibody, Clone: S164-6: FITC
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameCorin Antibody, KIAA0816 Antibody, LDLR Antibody, LDLR dan Antibody, Low density lipoprotein receptor related protein 4 Antibody, LRP-4 Antibody, LRP10 Antibody, MEGF7 Antibody, Multiple epidermal growth factor like domains 7 Antibody
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Raised inMouse
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Antibody s targetLRP4
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Primary research fieldsNeuroscience, Cell Structure, Membrane Markers, Cell Signaling
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BrandnameNone
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Antibodies applicationsWB, ICC/IF
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects the cytoplasmic domain of LRP4 ~215kDa, small degradation products ~100kD.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-415 was sufficient for detection of LRP4in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellMembrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextPotential cell surface endocytic receptor, which bindsand internalizes extracellular ligands for degradation by lysosomes. It is involved in the negative regulation of the canonical Wnt signaling pathway, being able to antagonize the LRP6-mediated activation of this pathway.
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Bibliography1. Bennett V., Baines A.J. (2001) Physiol. Rev. 81 (3): 1353–92.
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Released date27-Feb-2013
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_766256.3
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Gene number83469
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Protein numberQ8VI56
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-LRP4 (Cytoplasmic) Monoclonal Antibody, Clone S164-6 (SMC-415). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-LRP4 (Cytoplasmic) Monoclonal Antibody (SMC-415) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) LRP4 (Cytoplasmic) Antibody (D) Composite. Western Blot analysis of Rat Brain Membrane showing detection of ~215 kDa LRP4 protein using Mouse Anti-LRP4 Monoclonal Antibody, Clone S164-6 (SMC-415). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane. Load: 10 µg. Block: 5% milk. Primary Antibody: Mouse Anti-LRP4 Monoclonal Antibody (SMC-415) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~215 kDa. Mouse Anti-LRP4 (Cytoplasmic) Antibody [S164-6] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-415) Mouse Anti-LRP4 Antibody [S164-6] used in Western Blot (WB) on Rat Brain Membrane (SMC-415)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolLRP4-AS1, RBM25, LRP4, CORIN, MIR1302-6, PIRC28, PIRC24, PIRC26, PIRC27
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Short nameMouse Anti-Mouse LRP4 (Cytoplasmic) Monoclonal IgG1, Clone S164-6
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelFITC
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SpeciesMouse, Mouses
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Alternative nameMouse Antibody toMouse low density lipoprotein receptor-related protein 4 (Cytoplasmic) monoclonal IgG1, clonality S164-6
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Alternative techniquemurine, antibodies
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Alternative to gene targetlow density lipoprotein receptor-related protein 4, CLSS and LRP-4 and LRP10 and MEGF7 and SOST2, LRP4 and IDBG-42508 and ENSG00000134569 and 4038, scaffold protein binding, Cell surfaces, Lrp4 and IDBG-191212 and ENSMUSG00000027253 and 228357, BT.103632 and IDBG-637271 and ENSBTAG00000008429 and 504317
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Clone nameS164-6
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Gene info
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Identity
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Gene
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Long gene nameLRP4 antisense RNA 1
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Synonyms gene name
- LRP4 antisense RNA 1 (non-protein coding)
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Locus
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Discovery year2012-06-23
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Entrez gene record
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RefSeq identity
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA binding motif protein 25
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Synonyms gene
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Synonyms gene name
- RNA-binding region (RNP1, RRM) containing 7
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-12-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- RNA binding motif containing
- Spliceosomal A complex
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameLDL receptor related protein 4
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Synonyms gene name
- low density lipoprotein receptor-related protein 4
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-03-25
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Low density lipoprotein receptors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecorin, serine peptidase
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Synonyms gene name
- corin, serine protease
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-05-10
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Entrez gene record
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Pubmed identfication
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Classification
- Scavenger receptor cysteine rich domain containing
- Type II transmembrane serine proteases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1302-6
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1302 family
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 28
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 24
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 26
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 27
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
