IgG2a, Murine Negative Control (for rat tissue) (PE)

Products_type

Isotype Control
Products_short_name

[IgG2a, Murine Negative Control]
Clone

[3H1378]
Reactivity

Human, Rat
Specificity

Recognizes the human CD45 antigen. Species Crossreactivity: Negative on the following rat tissues-peripheral blood leucocytes, thymocytes, splenocytes and macrophages.
Purity

Highly Purified Purified by Ion Exchange chromatography.
Form

Supplied as a lyophilized powder in PBS, pH 7.2, 0.1% sodium azide, 1% BSA. Labeled with R. Phycoerythrin (RPE). Reconstitute with 1ml distilled
Storage_stability

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Description

Isotype or positive controls by peptides, antibodies and deactivated samples. Negative control peptides or isotypes available of the different species. 6
About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name

Rattus norvegicus

Gene target

IgG2a Murine for tissue
Short name

IgG2a, Murine Negative Control (for tissue) (PE)
Technique

Negative, Control, tissue, controls, negatif, tissues
Host

mouse
Isotype

IgG2a, IgG2a
Label

PE
Species

Rat, Rats
Alternative name

IgG2a, Murine Negative reference (to measure rat tissue) (peroxidase)
Alternative technique

controls, tissues
Tissue

control, tissue

Name

Negative Staining
Concept

Scope note: The technique of washing tissue specimens with a concentrated solution of a heavy metal salt and letting it dry. The specimen will be covered with a very thin layer of the metal salt, being excluded in areas where an adsorbed macromolecule is present. The macromolecules allow electrons from the beam of an electron microscope to pass much more readily than the heavy metal; thus, a reversed or negative image of the molecule is created.
Tree numbers
- E01.370.225.500.620.670.520
- E01.370.225.750.600.670.520
- E05.200.500.620.670.520
- E05.200.750.600.670.520
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data