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Category
Primary Antibodies
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Long description
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 Ð 7.8. The individual human cytokeratins are numbered 1 to 20._x000B_The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium._x000B_Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using_x000B_immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
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Antibody come from
Human transmembrane leukocyte antigen related PTPCatalytic domain, (a.a.1330-1620) (Gene bank accession number NM_130440) with Nterminal GST tag, MW=58 kDa, expressed in an E.coli expression system
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Other description
25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT. Protein A/G Chromatography
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Clone
RCK105
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Antigen antibody binding interaction
Mouse anti Cytokeratin 7 Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human, Mouse, Rat, Hamster, Canine, Swine, Feline
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry
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Antibody s suited for
Useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates. Specific Activity: 0.7 U/ug. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 37¡C. Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, and 100 mM NaCl. Enzyme reaction condition: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3mM DTT, 100 mM NaCl, 20 mM pNPP, and 160 ng/µl enzyme, for 5 min at 30¡C.
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Storage
-20ºC
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Relevant references
1.H. Cho et al. Biochemistry 1991, 30: 6210-6216_x000B__x000B_2.H. Cho et al. Biochemistry 1992, 31: 133-138
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
