Annexin V-PE Apoptosis Kit Plus

• Catalog number
  K203-400
• Price
  Please ask
• Size
  400 assays

• Description
  Detects Apoptotic, Necrotic & Healthy cells within 10 min
• Summary
  • Detection method- Flow cytometry using FL1 channel for SYTOX Green dye (Ex = 488 nm; Em = 530 nm) and FL2 channel for Annexin V-PE (Ex = 488 nm; Em = 578 nm). • Species reactivity- Mammalian • Kit size- Convenient sizes (25, 100 and 400 assays) • Applications- Detecting apoptosis in living cells by flow cytometry or fluorescence microscopy. PE shows brighter red fluorescence. Sytox green dye is also included to control necrosis.
• Sample Type
  Live cells
• Features Benefits
  • Simple one-step procedure; takes only 10 minutes • Fast and convenient • Apoptotic cells show red fluorescence, dead cells show green fluorescence and live cells show little or no fluorescence.
• Storage Conditions
  +4°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  The Annexin V-PE Apoptosis Detection Kit Plus is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily be detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need of fixation. The Annexin V-PE Apoptosis Detection Kit Plus includes annexin V-PE, SYTOX green dye, and binding buffer. The SYTOX green dye is impermeable to live cells and apoptotic cells, but stains necrotic cells with intense green fluorescence by binding to cellular nucleic acids. After staining a cell population with annexin V-PE and SYTOX Green dye in the provided binding buffer, apoptotic cells show red fluorescence, dead cells show green fluorescence and live cells show little or no fluorescence. These populations can easily be distinguished by Fluorescence microscopy using FITC and PE filters or by flow cytometry using the FL1 channel (Ex. 488 nm/Em. 530 nm) for SYTOX Green dye and FL2 channel for Annexin V-PE (Ex. 488 nm/Em. 578 nm).
• Data Sheet Link
• Additional description
  This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Biovision has more cell death kits and antibodies. This 1 is suited for programmed cell-death studies.

• Gene target
  Annexin   V-PE   Kit   Plus  
• Gene symbol
  ETV3, GH2, ERF, POLH, B4GALT5, IGLVV-66, MGAT5, IGLVV-58, HLA-V, APOA5
• Short name
  Annexin V-PE Apoptosis Kit Plus
• Technique
  apoptosis, apoptosis pathway
• Label
  PE
• Alternative name
  Annexin V-peroxidase programmed cellular destruction reagent Plus
• Alternative technique
  celldeath, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:3492
• Gene
  ETV3
• Long gene name
  ETS variant transcription factor 3
• Synonyms gene name
  • ets variant gene 3, ETS family transcriptional repressor
  • ETS variant 3
• Synonyms
  • PE-1
• GenBank acession
  • BC022868
• Locus
  1q23.1
• Discovery year
  1994-12-15
• Entrez gene record
  2117
• Pubmed identfication
  • 8020980
• RefSeq identity
  • NM_005240
• Classification
  • ETS transcription factor family
• VEGA ID
  OTTHUMG00000034298

Gene info

• Identity
  HGNC:4262
• Gene
  GH2
• Long gene name
  growth hormone 2
• Synonyms
  • GH-V
  • GHV
  • GHL
  • hGH-V
• Synonyms name
  • placental-specific growth hormone
  • placenta-specific growth hormone
• GenBank acession
  • J03756
• Locus
  17q23.3
• Discovery year
  2001-06-22
• Entrez gene record
  2689
• Pubmed identfication
  • 6306568
• RefSeq identity
  • NM_002059
• Classification
  • Growth hormone family
• VEGA ID
  OTTHUMG00000172284

Gene info

• Identity
  HGNC:3444
• Gene
  ERF
• Long gene name
  ETS2 repressor factor
• Synonyms gene name
  • Ets2 repressor factor
• Synonyms
  • PE-2
  • PE2
• GenBank acession
  • U58535
• Locus
  19q13.2
• Discovery year
  1998-07-17
• Entrez gene record
  2077
• Pubmed identfication
  • 7588608
  • 9192842
• RefSeq identity
  • NM_006494
• Classification
  • ETS transcription factor family
• VEGA ID
  OTTHUMG00000182788

Gene info

• Identity
  HGNC:9181
• Gene
  POLH
• Long gene name
  DNA polymerase eta
• Synonyms gene name
  • polymerase (DNA directed), eta
  • polymerase (DNA) eta
• Synonyms
  • RAD30A
  • XP-V
• GenBank acession
  • AB024313
• Locus
  6p21.1
• Discovery year
  1997-09-12
• Entrez gene record
  5429
• Pubmed identfication
  • 20012583
  • 10385124
  • 10398605
• RefSeq identity
  • NM_006502
• Classification
  • DNA polymerases
  • Xeroderma pigmentosum complementation groups
• VEGA ID
  OTTHUMG00000014743
• Locus Specific Databases
  • LRG_470

Gene info

• Identity
  HGNC:928
• Gene
  B4GALT5
• Long gene name
  beta-1,4-galactosyltransferase 5
• Synonyms gene name
  • UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5
• Synonyms
  • beta4GalT-V
• Synonyms name
  • beta4-GalT IV
• GenBank acession
  • AB004550
• Locus
  20q13.13
• Discovery year
  1999-03-15
• Entrez gene record
  9334
• Pubmed identfication
  • 9597550
  • 9435216
• RefSeq identity
  • NM_004776
• Classification
  • Beta 4-glycosyltransferases
• VEGA ID
  OTTHUMG00000033086

Gene info

• Identity
  HGNC:5946
• Gene
  IGLVV-66
• Long gene name
  immunoglobulin lambda variable (V)-66 (pseudogene)
• Synonyms gene name
  • immunoglobulin lambda variable (V)-66
  • immunoglobulin lambda variable (V)-66 pseudogene
• Synonyms
  • IGLV(V)-66
• GenBank acession
  • D87004
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28757
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151584

Gene info

• Identity
  HGNC:7049
• Gene
  MGAT5
• Long gene name
  alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase
• Synonyms gene name
  • mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase
• Synonyms
  • GNT-V
  • MGAT5A
• Synonyms name
  • alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
• GenBank acession
  • D17716
• Locus
  2q21.2-q21.3
• Discovery year
  1994-07-26
• Entrez gene record
  4249
• Pubmed identfication
  • 8292036
• RefSeq identity
  • NM_002410
• Classification
  • Mannosyl-glycoprotein N-acetylglucosaminyltransferases
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000131681

Gene info

• Identity
  HGNC:5945
• Gene
  IGLVV-58
• Long gene name
  immunoglobulin lambda variable (V)-58 (pseudogene)
• Synonyms gene name
  • immunoglobulin lambda variable (V)-58
  • immunoglobulin lambda variable (V)-58 pseudogene
• Synonyms
  • IGLV(V)-58
• GenBank acession
  • D87000
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28758
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000150893

Gene info

• Identity
  HGNC:23482
• Gene
  HLA-V
• Long gene name
  major histocompatibility complex, class I, V (pseudogene)
• Synonyms gene
  • HLA-75
• Synonyms gene name
  • HLA-75 pseudogene
• Synonyms
  • dJ377H14.4
• GenBank acession
  • M96332
• Locus
  6p22.1
• Discovery year
  2004-01-16
• Entrez gene record
  352962
• RefSeq identity
  • NR_132323
• Classification
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031277
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:17288
• Gene
  APOA5
• Long gene name
  apolipoprotein A5
• Synonyms gene name
  • apolipoprotein A-V
• Synonyms
  • RAP3
  • APOA-V
• GenBank acession
  • AF202889
• Locus
  11q23.3
• Discovery year
  2001-12-11
• Entrez gene record
  116519
• Pubmed identfication
  • 11588264
  • 11577099
• Classification
  • Apolipoproteins
• VEGA ID
  OTTHUMG00000046116

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