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Immunogen
A phospho-specific peptide corresponding to residues surrounding Ser435 and Ser436 of human ATR (AA432-439)
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Antibody s target
Human ATR (pSer435 + pSer436)
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Antibody s full description
Rabbit Anti-Human ATR (pSer435 + pSer436) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
FRAP Related Protein 1 Antibody, MEC1 Antibody, RAC3 Antibody, SCKL Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 301.367 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-918 was sufficient for detection of ATR (pSer435 + pSer436) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Nucleus, PML body
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Tissue specificity
Ubiquitous
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Scientific context
ATR is a protein-serine/threonine kinase. It is part of the PI3K family and functions in a complex with ATRIP. It responds to DNA damage and replication stresses and activates proteins that mediate DNA repair such as p53, BRCA1 and more. It other roles involve checkpoint signalling by interacting with chk1, fragile site stabilty and regulation of centrosome duplication.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_001175
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Gene number
545
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Protein number
Q13535
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Biotinylated
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
ANTXR1, SERPINA2, ATR
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Short name
Rabbit Anti- ATR (pSer435 + pSer436) Polyclonal:
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
Biotin
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman ataxia telangiectasia and Rad3 related (pSer435 + pSer436) polyclonal: biotinilated
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
ataxia telangiectasia and Rad3 related, FCTCS and FRP1 and MEC1 and SCKL and SCKL1, ATR and IDBG-59521 and ENSG00000175054 and 545, transferase activity, nuclei, Atr and IDBG-191646 and ENSMUSG00000032409 and 245000, ATR and IDBG-638174 and ENSBTAG00000011529 and 504869
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