COMBI Surface: IgG2a Negative Control (FITC) and IgG1 Negative Control (PE), Negative Control

• Catalog number
  GCT-202
• Price
  Please ask
• Size
  50Tests

• Host Source
  NA
• Clone Name
  4H1-A7 and VI-AP
• Product Category
  NA
• Species Reactivity
  Mouse
• Application
  Direct Immunofluorescence
• Immunogen
  NA
• Purification Method
  NA
• Shipping Conditions
  NA
• Storage conditions
  COMBI Surface: IgG2a Negative Control (FITC) and IgG1 Negative Control (PE), Negative Control MUbio monoclonal antibody reagents contain optimal concentrations of affinity-purified antibody. For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8ÁC (DO NOT FREEZE!) and protected from prolonged exposure to light. Stability of the reagent: Please refer to the expiry date printed onto the vial. The use of the reagent after the expiration date is not recommended. _x000B_
• Datasheet link
  NA
• Description
  Isotype or positive controls by peptides, antibodies and deactivated samples. Negative control peptides or isotypes available of the different species.
• Properties
  This Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody

• Gene target
  COMBI   Surface   IgG2a   IgG1  
• Short name
  COMBI Surface: IgG2a Negative Control (FITC) IgG1 Negative Control (PE), Negative Control
• Technique
  Negative, Control, FITC, controls, Fluorescein, negatif
• Isotype
  IgG2a and IgG1, IgG1, IgG2a
• Label
  FITC
• Alternative name
  COMBI Surface: IgG2a Negative reference (fluorecein) and IgG1 Negative reference (peroxidase), Negative reference
• Alternative technique
  controls, fluorescine
• Tissue
  control

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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