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Clone
S112B-14
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Immunogen
Fusion protein amino acids 41-64 and 189-428 of mouse Kir2.1
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Antibody s full description
Mouse Anti-Mouse Kir2.1 Monoclonal IgG1 Antibody, Clone: S112B-14: ATTO 655
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
HHBIRK1 Antibody, HHIRK1 Antibody, HIRK 1 Antibody, IRK1 Antibody, KCNJ2 Antibody, LQT7 Antibody, SQT3 Antibody, potassium inwardly rectifying channel J2 Antibody
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Raised in
Mouse
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Antibody s target
Kir2.1
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Primary research fields
Neuroscience, Ion Channels, Potassium Channels, Inward-Rectifying Potassium Channels
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Brandname
None
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Antibodies applications
WB, IHC, ICC/IF, AM
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Antibody s reactivity
Human, Mouse, Rat, Monkey
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Antibody s dilutions
WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~45kDa. No cross-reactivity against Kir2.2 or Kir2.3.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml of SMC-310 was sufficient for detection of Kir2.1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Membrane
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
The Kir2.1 inward-rectifier potassium ion channel is encoded by the KCNJ2 gene. A defect in this gene is associated with Andersen-Tawil syndrome (1).
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Bibliography
1. Donaldson M.R., Yoon G., Fu Y.H., Ptacek L.J. (2004). Ann. Med. 36 Suppl 1: 92–7.
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Released date
15-Aug-2009
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_032451
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Gene number
16518
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Protein number
P35561
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112B-14 (SMC-310). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody (SMC-310) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear expression in the epidermis and hair follicles. Western Blot analysis of Monkey COS transient cell lysate showing detection of Kir2.1 Potassium Channel protein using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112B-14 (SMC-310). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody (SMC-310) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunohistochemistry analysis using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112B-14 (SMC-310). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody (SMC-310) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Mouse Anti-Kir2.1 Potassium Channel Antibody [S112B-14] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-310) Mouse Anti-Kir2.1 Potassium Channel Antibody [S112B-14] used in Western Blot (WB) on Monkey COS transient cell lysate (SMC-310) Mouse Anti-Kir2.1 Potassium Channel Antibody [S112B-14] used in Immunohistochemistry (IHC) on Human hippocampus (SMC-310)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
