RAT ANTI MOUSE JAM-C:FITC Antibody

• Catalog number
  GEN224532
• Price
  Please ask
• Size
  0.025 miligrams

• Also known as
  JAM-C
• Other names
  junctional adhesion molecule C; Junctional adhesion molecule C; junctional adhesion molecule C; junctional adhesion molecule 3; junction cell adhesion molecule 3; junction cell adhesion molecule C; junction adhesion molecule 3; JAM-2; Junctional adhesion molecule 3
• Category
  Antibodies
• Subcategory
  Mnoclonal antibodies
• Gene name
  JAM-C
• Gene name synonims
  N/A
• Other gene names
  Jam3; Jam3; JAM-3; JAM-C; Jcam3; 1110002N23Rik; JAM-C; JAM-3
• Clonality
  Monoclonal
• Immunoglobulin isotype
  IgG2a
• Clone
  CRAM-19 H36
• Host organism
  Rat (Rattus norvegicus)
• Species reactivity
  Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
• Specificity and cross reactivity
  JAM-C Clone CRAM-19 H36 recognises mouse and human Junctional adhesion molecule C (JAM-C), also known as JAM-3 and, historically, as JAM-2. JAM-C is expressed at junctions between endothelial and epithelial cells, as well as on leukocytes, platelets, vascular smooth muscle cells and fibroblasts, amongst other cell types. It plays a role in tight junctions and inflammatory processes and interacts with JAM-A and JAM-B.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
• Purification method
  N/A
• Form Appearance
  FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
• Concentration
  IgG concentration 0.1 miligram per 1 mililiter
• Storage and shipping
  Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term.Storage in frost-free freezers is not recommended. the antibody should be stored undiluted. the antibody is photosensitive and should be protected from light. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
• Tested applications
  Immunofluorescence (IF), Flow Cytometry (FACS)
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
• Properties
  If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus, Rattus norvegicus
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• French translation
  anticorps

• Gene target
  JAM-C  
• Gene symbol
  JAM3, JAM2, F11R, SMC4, PCK1, MYB, SFTPC, VCX3B, MAF, RFX1
• Short name
  ANTI MOUSE JAM-C:FITC Antibody
• Technique
  anti mouse, Antibody, Mouse, anti, FITC, antibody to, anti mouse antibodies, antibodies against human proteins, antibodies for, Fluorescein, mouses
• Host
  Rat
• Label
  FITC
• Species
  Mouse, Mouses, Rats
• Alternative name
  Anti-RAT ANTI MOUSE JAM-C:FITC
• Alternative technique
  antibodies, murine, fluorescine

Gene info

• Identity
  HGNC:15532
• Gene
  JAM3
• Long gene name
  junctional adhesion molecule 3
• Synonyms
  • JAM-C
  • JAMC
• GenBank acession
  • AF356518
• Locus
  11q25
• Discovery year
  2001-04-26
• Entrez gene record
  83700
• RefSeq identity
  • NM_032801
• Classification
  • Small nucleolar RNA protein coding host genes
  • Ig-like cell adhesion molecule family
  • V-set domain containing
• VEGA ID
  OTTHUMG00000167130

Gene info

• Identity
  HGNC:14686
• Gene
  JAM2
• Long gene name
  junctional adhesion molecule 2
• Synonyms gene
  • C21orf43
• Synonyms
  • VE-JAM
  • JAM-B
  • JAMB
  • CD322
• GenBank acession
  • AF255910
• Locus
  21q21.3
• Discovery year
  2001-04-25
• Entrez gene record
  58494
• Pubmed identfication
  • 10779521
  • 10945976
• Classification
  • CD molecules
  • Ig-like cell adhesion molecule family
  • I-set domain containing
  • V-set domain containing
• VEGA ID
  OTTHUMG00000078441

Gene info

• Identity
  HGNC:14685
• Gene
  F11R
• Long gene name
  F11 receptor
• Synonyms gene
  • JAM1
• Synonyms gene name
  • junctional adhesion molecule 1
• Synonyms
  • PAM-1
  • JCAM
  • JAM-1
  • JAM-A
  • JAMA
  • CD321
• GenBank acession
  • AF111713
• Locus
  1q23.3
• Discovery year
  2001-04-10
• Entrez gene record
  50848
• Pubmed identfication
  • 10395639
  • 7646439
• RefSeq identity
  • NM_016946
• Classification
  • Ig-like cell adhesion molecule family
  • V-set domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000028602

Gene info

• Identity
  HGNC:14013
• Gene
  SMC4
• Long gene name
  structural maintenance of chromosomes 4
• Synonyms gene
  • SMC4L1
• Synonyms gene name
  • SMC4 (structural maintenance of chromosomes 4, yeast)-like 1
  • SMC4 structural maintenance of chromosomes 4-like 1 (yeast)
• Synonyms
  • hCAP-C
  • CAP-C
• GenBank acession
  • AF092564
• Locus
  3q25.33
• Discovery year
  2001-02-22
• Entrez gene record
  10051
• Pubmed identfication
  • 9789013
  • 10319587
• Classification
  • Structural maintenance of chromosomes proteins
  • Condensin I subunits
  • Condensin II subunits
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000159006

Gene info

• Identity
  HGNC:8724
• Gene
  PCK1
• Long gene name
  phosphoenolpyruvate carboxykinase 1
• Synonyms gene name
  • phosphoenolpyruvate carboxykinase 1 (soluble)
• Synonyms
  • PEPCK-C
• Locus
  20q13.31
• Discovery year
  1990-09-10
• Entrez gene record
  5105
• Pubmed identfication
  • 1492743
• VEGA ID
  OTTHUMG00000032825

Gene info

• Identity
  HGNC:7545
• Gene
  MYB
• Long gene name
  MYB proto-oncogene, transcription factor
• Synonyms gene name
  • v-myb avian myeloblastosis viral oncogene homolog
• Synonyms
  • c-myb
• Locus
  6q23.3
• Discovery year
  2001-06-22
• Entrez gene record
  4602
• Pubmed identfication
  • 17599807
• Classification
  • Myb/SANT domain containing
• VEGA ID
  OTTHUMG00000015629
• Locus Specific Databases
  • The Globin Gene Server

Gene info

• Identity
  HGNC:10802
• Gene
  SFTPC
• Long gene name
  surfactant protein C
• Synonyms gene
  • SFTP2
• Synonyms gene name
  • surfactant, pulmonary-associated protein C
• Synonyms
  • SP-C
  • PSP-C
  • SMDP2
  • BRICD6
• Synonyms name
  • BRICHOS domain containing 6
• Locus
  8p21.3
• Discovery year
  1988-05-11
• Entrez gene record
  6440
• Pubmed identfication
  • 1859376
  • 16709565
• RefSeq identity
  • NM_003018
• Classification
  • BRICHOS domain containing
• VEGA ID
  OTTHUMG00000163775

Gene info

• Identity
  HGNC:31838
• Gene
  VCX3B
• Long gene name
  variable charge X-linked 3B
• Synonyms
  • VCX-C
• Locus
  Xp22.31
• Discovery year
  2005-01-11
• Classification
  • Variable charge X/Y family
• VEGA ID
  OTTHUMG00000021106

Gene info

• Identity
  HGNC:6776
• Gene
  MAF
• Long gene name
  MAF bZIP transcription factor
• Synonyms gene name
  • v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog
• Synonyms
  • c-MAF
• Locus
  16q23.2
• Discovery year
  1991-08-01
• Entrez gene record
  4094
• Pubmed identfication
  • 14998484
• Classification
  • Basic leucine zipper proteins
• VEGA ID
  OTTHUMG00000137621

Gene info

• Identity
  HGNC:9982
• Gene
  RFX1
• Long gene name
  regulatory factor X1
• Synonyms gene name
  • regulatory factor X, 1 (influences HLA class II expression)
• Synonyms
  • EF-C
• Synonyms name
  • trans-acting regulatory factor 1
  • enhancer factor C
  • MHC class II regulatory factor RFX
• Locus
  19p13.12
• Discovery year
  1991-11-06
• Entrez gene record
  5989
• Pubmed identfication
  • 1505960
  • 8289803
• RefSeq identity
  • NM_002918
• Classification
  • Regulatory factor X family
• VEGA ID
  OTTHUMG00000181979

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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