COMBI IC Reagent: Mouse anti Myeloperoxidase-C2 (FITC) and Mouse anti CD22 (PE)

• Catalog number
  GIC-214
• Price
  Please ask
• Size
  50 Tests

• Category
  Primary Antibodies
• Long description
  Myeloperoxidase (MPO) is a glycoprotein present in the azurophil (primary) granules of myeloid cells, which appears in the myeloblast stage of myeloid cell differentiation. MPO is he most common functional protein of myeloid cells and is involved in the inflammatory response. It helps to kill microbes by breaking down peroxide in the presence of halide ions, contributing to the bactericidal function of granulocytes. The primary translation product of MPO undergoes glycosylation with production of the 89 kDa heme-free apopro-MPO form followed by incorporation of heme and conversion into the enzymatically active pro-MPO form. Subsequently, pro-MPO becomes targeted to azurophil granules where final processing occurs to produce mature dimeric MPO consisting of the 59-64 kDa MPO α-chain and the 14 kDa MPO β-chain. Precursor B-cells are surface-CD22 negative, but cytoplasmic CD22 positive. Mature B-lymphocytes.express CD22 also on their surface. The combined staining for MPO and CD22 allows the distinction of mature/immature myeloid cells and B-lymphocytes. The MPO-C2/CD22 COMBI-IC mAb permits the identification and enumeration of normal and malignant myelomonocytic cells and B lymphoid commited cells in human blood and bone marrow using flow cytometry. Results must be put within the context of other diagnostic tests as well as the clinical history of the patient by a certified professional before final interpretation. Analyses performed with this antibody should be paralleled by positive and negative controls. If unexpected results are obtained which cannot be attributed to differences in laboratory procedures, please contact us.
• Antibody come from
  n/a
• Other description
  PBS pH 7.2, 1 mg/ml BSA, 0.1% NaN3
• Clone
  8E6 and RFB4
• Antigen antibody binding interaction
  COMBI IC Reagent: Mouse anti Myeloperoxidase-C2 (FITC) and Mouse anti CD22 (PE) Antibody
• Antibody is raised in
  Mouse
• Antibody s reacts with
  Human
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  Antibody MPO-C2 (clone 8E2) reacts with human myeloperoxidase (MPO) expressed by normal and malignant myelomonocytic cells. The CD22 mAb (clone RFB4) recognizes cytoplasmatic CD22 in precursor B-cells and surface as well cytoplasmatic CD22 on mature B-cells. In this COMBI-IC Reagent antibody 8E6 is conjugated to FITC, antibody RFB4 is conjugated to Phycoeythrin (PE). The sensitivity of MPO-C2/CD22 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity). For this purpose, a mAb-concentration range is selected to include both the saturation point (i.e. the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i.e. the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells). In practice, 50 µl of leukocytes containing 10^7 cells/ml are stained with 20µl mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity.
• Research interest
  CD Marker
• Application
  Flow cytometry
• Antibody s suited for
  Permeabilization and Staining Procedure - In combination with our Permeabilization Kit FIX&PERM (Cat. No. GAS-002) intracellular MPO-C2 and CD22 can be easily stained in cell suspensions. - For each sample to be analyzed add 50 µl of whole blood, bone marrow or mononuclear cell suspension in a 5ml tube - Add 100 µl of Reagent A (Fixation Medium, stored and used at room temperature) - Incubate for 15 minutes at room temperature - Add 5ml phosphate buffered saline and centrifuge cells for 5 minutes at 300 g - Remove supernatant and add to cell pellet 100 µl Reagent B (Permeabilization Medium) and 20 µl of the MPO-C2/CD22 COMBI-IC monoclonal antibody conjugate - Vortex at low speed for 1-2 seconds - Incubate for 15 minutes at room temperature - Wash cells with phosphate buffered saline as described above - Remove supernatant and resuspend cells in sheath fluid for immediate analysis or resuspend cells in 0.5 ml 1.0 % formaldehyde and store them at 2-8°C in the dark. Analyze fixed cells within 24 hours.
• Storage
  Nordic-MUbio monoclonal antibody reagents contain optimal concentrations of affinity-purified antibody. For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8°C (DO NOT FREEZE!) and protected from prolonged exposure to light. Stability of the reagent: Please refer to the expiry date printed onto the vial. The use of the reagent after the expiration date is not recommended.
• Relevant references
  Andersson, E., Hellman, L., Gullberg, U. & Olsson, I. (1998) J Biol Chem 273, 4747-53. Beverley, P. C., Linch, D. & Callard, R. E. (1981) Haematol Blood Transfus 26, 309-13. Boue, D. R. & Lebien, T. W. (1988) J Immunol 140, 192-9. Braylan, R. C., Orfao, A., Borowitz, M. J. & Davis, B. H. (2001) Cytometry 46, 23-7 Catovsky, D., Matutes, E., Buccheri, V., Shetty, V., Hanslip, J., Yoshida, N. & Morilla, R. (1991) Ann Hematol 62, 16-21. Janossy, G., Coustan-Smith, E. & Campana, D. (1989) Leukemia 3, 170-81. Knapp, W., Majdic, O. & Strobl, H. (1993) Recent Results Cancer Res 131, 31-40. Koeffler, H. P., Ranyard, J. & Pertcheck, M. (1985) Blood 65, 484-91. Konikova, E., Glasova, M., Kusenda, J. & Babusikova, O. (1998) Neoplasma 45, 282-91. Lanza, F., Latorraca, A., Moretti, S., Castagnari, B., Ferrari, L. & Castoldi, G. (1997) Cytometry 30, 134-44. Li, J. L., Shen, G. L., Ghetie, M. A., May, R. D., Till, M., Ghetie, V., Uhr, J. W., Janossy, G., Thorpe, P. E., Amlot, P. & et al. (1989) Cell Immunol 118, 85-99. Mason, D. Y., Stein, H., Gerdes, J., Pulford, K. A., Ralfkiaer, E., Falini, B., Erber, W. N., Micklem, K. & Gatter, K. C. (1987) Blood 69, 836-40. Murao, S., Stevens, F. J., Ito, A. & Huberman, E. (1988) Proc Natl Acad Sci U S A 85, 1232-6.
• Protein number
  see ncbi
• Warnings
  For professional users only. This reagent contains sodium azide. To avoid the development of hazardous conditions, reagents containing azide should be diluted in running water prior to be discarded. Similar to the work with other biological products, proper handling procedures are recommended.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Properties
  This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  COMBI   Reagent   Myeloperoxidase-C2   CD22  
• Gene symbol
  C2, SNAR-C2, C2-AS1, PCDHAC2, GCNT3, NPC2, PIK3C2B, MAGEC2, CD22
• Short name
  COMBI IC Reagent: Mouse anti Myeloperoxidase-C2 (FITC) Mouse anti CD22 (PE)
• Technique
  Mouse, anti, FITC, antibody to, Fluorescein, mouses
• Host
  mouse
• Isotype
  IgG1
• Label
  FITC and PE
• Species
  Mouse, Mouses
• Alternative name
  COMBI IC Reagent: Mouse antibody to Myeloperoxidase-complement component 2 (fluorecein) and Mouse antibody to CD22 molecule (peroxidase)
• Alternative technique
  murine, antibodies, fluorescine
• Alternative to gene target
  CD22 molecule, SIGLEC-2 and SIGLEC2, CD22 and IDBG-44001 and ENSG00000012124 and 933, protein binding, Cell surfaces, Cd22 and IDBG-171574 and ENSMUSG00000030577 and 12483, CD22 and IDBG-635610 and ENSBTAG00000011887 and 514582

Gene info

• Identity
  HGNC:1248
• Gene
  C2
• Long gene name
  complement C2
• Synonyms gene name
  • complement component 2
• Locus
  6p21.33
• Discovery year
  2001-06-22
• Entrez gene record
  717
• Classification
  • Complement system activation components
  • Sushi domain containing
• VEGA ID
  OTTHUMG00000031190
• Locus Specific Databases
  • C2base: Mutation registry for C2 deficiency
  • LRG_26

Gene info

• Identity
  HGNC:34320
• Gene
  SNAR-C2
• Long gene name
  small NF90 (ILF3) associated RNA C2
• Synonyms gene name
  • small ILF3/NF90-associated RNA C2
• GenBank acession
  • EU071067
• Locus
  19q13.32
• Discovery year
  2008-07-03
• Entrez gene record
  100170218
• Pubmed identfication
  • 17855395
• RefSeq identity
  • NR_024217
• Classification
  • Small NF90 (ILF3) associated RNAs

Gene info

• Identity
  HGNC:49464
• Gene
  C2-AS1
• Long gene name
  C2 antisense RNA 1
• GenBank acession
  • BX100594
• Locus
  6p21.33
• Discovery year
  2013-12-06
• Entrez gene record
  102060414
• RefSeq identity
  • NR_104191
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000189556

Gene info

• Identity
  HGNC:8677
• Gene
  PCDHAC2
• Long gene name
  protocadherin alpha subfamily C, 2
• Synonyms
  • PCDH-ALPHA-C2
• GenBank acession
  • AF152474
• Locus
  5q31.3
• Discovery year
  2000-06-28
• Entrez gene record
  56134
• Pubmed identfication
  • 10380929
• RefSeq identity
  • NM_018899
• Classification
  • Clustered protocadherins
• VEGA ID
  OTTHUMG00000129607

Gene info

• Identity
  HGNC:4205
• Gene
  GCNT3
• Long gene name
  glucosaminyl (N-acetyl) transferase 3, mucin type
• Synonyms
  • C2GnT-M
  • C2/4GnT
  • C2GnT2
• GenBank acession
  • AF102542
• Locus
  15q22.2
• Discovery year
  1999-02-26
• Entrez gene record
  9245
• Pubmed identfication
  • 9915862
  • 9988682
• RefSeq identity
  • NM_004751
• Classification
  • Glucosaminyl (N-acetyl) transferases/xylosyltransferases
• VEGA ID
  OTTHUMG00000132726

Gene info

• Identity
  HGNC:14537
• Gene
  NPC2
• Long gene name
  NPC intracellular cholesterol transporter 2
• Synonyms gene name
  • Niemann-Pick disease, type C2
• Synonyms
  • HE1
  • NP-C2
  • EDDM1
• Synonyms name
  • epididymal protein 1
• GenBank acession
  • X67698
• Locus
  14q24.3
• Discovery year
  2001-05-11
• Entrez gene record
  10577
• Pubmed identfication
  • 8418812
  • 11125141
• RefSeq identity
  • NM_006432
• Classification
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000171212

Gene info

• Identity
  HGNC:8972
• Gene
  PIK3C2B
• Long gene name
  phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta
• Synonyms gene name
  • phosphoinositide-3-kinase, class 2, beta polypeptide
  • phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 beta
• Synonyms
  • C2-PI3K
  • PI3K-C2beta
• GenBank acession
  • Y11312
• Locus
  1q32.1
• Discovery year
  1998-05-21
• Entrez gene record
  5287
• Pubmed identfication
  • 9144573
  • 9830063
• RefSeq identity
  • NM_002646
• Classification
  • Phosphatidylinositol 3-kinase subunits
• VEGA ID
  OTTHUMG00000036101

Gene info

• Identity
  HGNC:13574
• Gene
  MAGEC2
• Long gene name
  MAGE family member C2
• Synonyms gene
  • MAGEE1
• Synonyms gene name
  • melanoma antigen, family E, 1, cancer/testis specific
  • melanoma antigen family C2
• Synonyms
  • CT10
  • MAGE-C2
  • HCA587
• Synonyms name
  • cancer/testis antigen 10
• GenBank acession
  • AF116195
• Locus
  Xq27.2
• Discovery year
  2000-09-26
• Entrez gene record
  51438
• Pubmed identfication
  • 10699956
  • 20864041
• RefSeq identity
  • NM_016249
• Classification
  • MAGE family
• VEGA ID
  OTTHUMG00000022574
• Locus Specific Databases
  • Global Variome shared LOVD

Gene info

• Identity
  HGNC:1643
• Gene
  CD22
• Long gene name
  CD22 molecule
• Synonyms gene name
  • CD22 antigen
• Synonyms
  • SIGLEC-2
  • SIGLEC2
• Synonyms name
  • sialic acid binding Ig-like lectin 2
• GenBank acession
  • X52785
• Locus
  19q13.12
• Discovery year
  1991-06-06
• Entrez gene record
  933
• Pubmed identfication
  • 8496602
  • 1691828
• RefSeq identity
  • NM_001771
• Classification
  • CD molecules
  • MicroRNA protein coding host genes
  • C2-set domain containing
  • Sialic acid binding Ig like lectins
• VEGA ID
  OTTHUMG00000183298

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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