COMBI IC Reagent: Mouse anti Myeloperoxidase-C2 (FITC) and Mouse anti CD22 (PE)
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Catalog number
MBS570348
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Price
Please ask
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Size
1 mL
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Products_type
Antibody
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Products_short_name
[Myeloperoxidase-C2 (FITC) and CD22 (PE)]
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Clone
[8E6; RFB4]
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Reactivity
Human
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Specificity
Antibody MPO-C2 (clone 8E2) reacts with human myeloperoxidase (MPO) expressed by normal and malignant myelomonocytic cells. The CD22 mAb (clone RFB4) recognizes cytoplasmatic CD22 in precursor B-cells and surface as well cytoplasmatic CD22 on mature B-cells. In this COMBI-IC Reagent antibody 8E6 is conjugated to FITC, antibody RFB4 is conjugated to Phycoeythrin (PE). The sensitivity of MPO-C2/CD22 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity). For this purpose, a mAb-concentration range is selected to include both the saturation point (i.e. the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i.e. the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells). In practice, 50 ul of leukocytes containing 10^7 cells/ml are stained with 20ul mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity.
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Form
PBS pH 7.2, 1 mg/ml BSA, 0.1% NaN3
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Storage_stability
Monoclonal antibody reagents contain optimal concentrations of affinity-purified antibody. For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8 degree C (DO NOT FREEZE!) and protected from prolonged exposure to light. Stability of the reagent: Please refer to the expiry date printed onto the vial. The use of the reagent after the expiration date is not recommended.
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Tested_application
Flow Cytometry (FC/FACS)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
C2, SNAR-C2, C2-AS1, PCDHAC2, GCNT3, NPC2, PIK3C2B, MAGEC2, CD22
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Short name
COMBI IC Reagent: Mouse anti Myeloperoxidase-C2 (FITC) Mouse anti CD22 (PE)
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Technique
Mouse, anti, FITC, antibody to, Fluorescein, mouses
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Host
Mouse
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Isotype
IgG1
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Label
FITC
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Species
Mouse, Mouses
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Alternative name
COMBI IC Reagent: Mouse antibody to Myeloperoxidase-complement component 2 (fluorecein) and Mouse antibody to CD22 molecule (peroxidase)
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Alternative technique
murine, antibodies, fluorescine
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Alternative to gene target
CD22 molecule, SIGLEC-2 and SIGLEC2, CD22 and IDBG-44001 and ENSG00000012124 and 933, protein binding, Cell surfaces, Cd22 and IDBG-171574 and ENSMUSG00000030577 and 12483, CD22 and IDBG-635610 and ENSBTAG00000011887 and 514582
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Gene info
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Identity
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Gene
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Long gene name
complement C2
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Synonyms gene name
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Locus
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Discovery year
2001-06-22
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Entrez gene record
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Classification
- Complement system activation components
- Sushi domain containing
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VEGA ID
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Locus Specific Databases
Gene info
Gene info
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Identity
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Gene
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Long gene name
C2 antisense RNA 1
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GenBank acession
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Locus
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Discovery year
2013-12-06
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Entrez gene record
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RefSeq identity
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Classification
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VEGA ID
Gene info
Gene info
Gene info
Gene info
Gene info
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Identity
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Gene
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Long gene name
MAGE family member C2
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Synonyms gene
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Synonyms gene name
- melanoma antigen, family E, 1, cancer/testis specific
- melanoma antigen family C2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
2000-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene name
CD22 molecule
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Synonyms gene name
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1991-06-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- MicroRNA protein coding host genes
- C2-set domain containing
- Sialic acid binding Ig like lectins
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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