SIRT5 (GST-tagged), Human recombinant
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Catalog number
7674-20
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Price
Please ask
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Size
20 ug
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Synonyms
SIRT5 (GST-tagged), Human recombinant
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Alternative_names
NAD-dependent Deacetylase 5; SIR2-like Protein 5; SIR2L5; Sirtuin 5; Silent Information Regulator 5
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Description
Intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥90%
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Assay
SDS-PAGE
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Molecular Weight
60.6 kDa (NT GST Tag), 26 kDa (native)
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Storage Temp
-80°C
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Shipping
dry ice
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Shelf Life
9 months
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Appearance
Liquid
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Physical form description
50 mM sodium phosphate, pH 7.2, 100 mM sodium chloride, and 20% glycerol.
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Background Information
The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) and have been shown to catalyze a reaction that couples lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose from NAD+ and the abstracted acetyl group. There are seven human sirtuins, which have been designated SIRT1-7. SIRT5 is located in the mitochondrial matrix and its functions are largely still being elucidated, however a few promising substrates have been studied. SIRT5 has been shown to deacetylate carbamoyl phosphate synthetase 1 (CSP1), activating the enzyme to catalyze the first step of the urea cycle. CSP1 is important in the detoxification of excess ammonia that can accumulate during fasting. Cytochrome C, another mitochondrial enzyme involved in oxidative metabolism can also be deacetylated by SIRT5. SIRT5 is predominantly found in the heart, brain, testis, and lymphoblasts.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
MGST3, SIRT5
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Short name
SIRT5 (GST-tagged), recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
SIRT5 (GST-tagged), H. sapiens Rec.
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Alternative technique
rec
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Gene info
Gene info
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Identity
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Gene
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Long gene name
sirtuin 5
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Synonyms gene name
- sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 5
- sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)
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GenBank acession
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Locus
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Discovery year
2001-03-20
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Entrez gene record
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Pubmed identfication
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Classification
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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