His-tag polyclonal antibody
#
-
Catalog numberAP0032-50ul
-
Price:
-
Size50ul
-
-
Alternative Name6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag
-
ImmunogenSynthetic peptide His-tag.
-
Swiss ProtN/A
-
ReactivityAll
-
ApplicationsWB
-
Application_allWB: 1:500~1:1000
-
ProductRabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
-
Purification PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
-
Storage StabilityStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
-
SpecificityHis-tag polyclonal antibody detects over-expressed or recombinant proteins containing the 6× His epitope tag
-
UsageWestern blot (WB) analysis of over-expressed His-tagged protein in E. coli, the antibody dilutions are 1:2000 (lane 1) and 1:5000 (lane 2). Each lane was loaded with 10 μg of E. coli lysate.
-
BiowMWN/A
-
NoteFor research use only, not for use in diagnostic procedure.
-
PropertiesIf you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
Conjugationhistidine
-
GroupPolyclonals and antibodies
-
AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
-
French translationanticorps
-
Gene target
-
Short nameHis-tag polyclonal antibody
-
TechniquePolyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope. Tags
-
HostRabbit
-
LabelHis
-
Alternative namehistidine-detection labelled polyclonal (antibody to-)
-
Alternative techniquepolyclonals, antibodies
-
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data