His Tag antibody HRP

Category

Primary Antibody
Antibody Subtype

Conjugated Polyclonal Antibodies
Area of research

Miscellaneous
Type of Immunogen

His Tag antibodies were raised in Rabbit using a 6-His tag conjugated to KLH .
Raised in

Rabbit
Isotypes

IgG
Conjugate Tag

HRP
Method of Purification

His Tag antibody (HRP) was purified by affinity chromatography
Concentration

1 mg/ml
Form Buffer

Provided in liquid form in PBS buffer containing 0.2% BSA and 0.1% Pro-Clean 400
Storage

Stable for 1 year from date of receipt at 2-8 deg C
Shipping conditions

Blue Ice
Tested for

ELISA; ICC; WB
Usage Recommendations

ELISA: 1:10,000-1:100,000, ICC: 1:200-1:500, WB: 1:1000-1:30,000
URL
More info
Properties

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
Conjugation

histidine, HRP Conjugated
French translation

anticorps

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data