Donkey anti-Sheep IgG (H&L) - Affinity Pure, FITC Conjugate, min x w/human or rabbit IgG[IgG (H&L)]

• Catalog number
  MBS687739
• Price
  Please ask
• Size
  NA

• Products_type
  Secondary Antibody
• Reactivity
  Sheep.Cross Reactivity: Based on IEP, no reactivity is observed to: non-immunoglobulin sheep serum proteins IgG from human or rabbit serum proteins from human or rabbit
• Test
  A high affinity purification column was use to purify anti- IgG (H L) - Pure, FITC Conjugate, min x w/ rabbit IgG[IgG (H L)] by MyBioSource by chromatographic size exclusion.
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The ultrapure anti- IgG (H L) - Pure, FITC Conjugate, min x w/ rabbit IgG[IgG (H L)] supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds.
• Latin name
  Equus africanus asinus, Oryctolagus cuniculus
• Properties
  This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Conjugation
  Anti-FITC Antibody
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

• Gene target
  Conjugate   min  
• Short name
  anti- IgG (H L) - Pure, FITC Conjugate, min x w/ rabbit IgG[IgG (H L)]
• Technique
  affinity, Rabbit, anti-, anti, FITC, Pure, IgG, antibody to, antibodies, Fluorescein, IgGs, pure products
• Host
  Donkey, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits. Rabbits
• Isotype
  IgG, Heavy & Light chains, IgG
• Label
  FITC
• Species
  Human, Donkeys, Humans, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
• Alternative name
  Donkey Antibody toSheep Immunoglobulin G (heavy + light chain) - protein Pure, fluorecein Conjugate, min x w/H. sapiens and production species: rabbit Immunoglobulin G[Immunoglobulin G (heavy + light chain)]
• Alternative technique
  rabbit-anti, antibodies, purified, fluorescine, immunoglobulins

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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