PCR DNA extraction and purification kit
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Catalog number
K1444-100
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Price
Please ask
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Size
100 test
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Description
DNA extraction and purification from PCR products
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Summary
PCR DNA extraction and purification kit provides a simple, rapid and efficient method for the recovery and purification of DNA directly from PCR products (100 bp to 50 kb) with typical recovery efficiency up to 85%.
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Applications
This kit provides a simple, rapid and efficient method for the recovery and purification of DNA directly from PCR products (100 bp to 50 kb) with typical recovery efficiency up to 85%.
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Sample Type
PCR products (100 bp to 50 kb)
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Features Benefits
kit can be used to concentrate DNA., The resulting product can be directly used for sequencing, restriction digestion, or PCR and other downstream experiments., Simple, rapid and efficient method
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Storage Conditions
RT and 2-8°C as described in the datasheet
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Shipping Conditions
RT
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Shelf life
12 months
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Background
PCR DNA extraction and purification kit provides a simple, rapid and efficient method for the recovery and purification of DNA directly from PCR products (100 bp to 50 kb) with typical recovery efficiency up to 85%. The resulting product can be directly used for sequencing, restriction digestion, or PCR and other downstream experiments. In addition, the kit can be used to concentrate DNA. The kit will work with a 96 well round bottom plates if a special magnetic frame is used. The kit can also be used with a variety of automatic nucleic acid extraction instruments and workstation.
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Group
PCR, polymerase chain reaction
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About
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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Properties
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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Test
Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. Biovision supplies purification kits and ultra pure reagents.
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Gene target
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Gene symbol
PRKDC, HLA-DOA
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Short name
PCR DNA extraction purification kit
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Technique
Purification, dna, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. purified
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Alternative name
PCR test kit Desoxyribonucleic acid extraction and purification reagent
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Alternative technique
purifications, kits, dna-amplification
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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