Rabbit anti Human IgG H + L Alk Phos
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Catalog number
43-I23
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Price
Please ask
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Size
1 mg
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Category
Secondary Antibody
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Antibody Subtype
Conjugated Secondary Antibodies
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Area of research
Immunology
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Type of Immunogen
Rabbit anti-human IgG (H+L) (Alk Phos) was raised in rabbit using human IgG whole molecule ..
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Raised in
Rabbit
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Specificity
Human IgG
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Cross Reactivity
Should be determined by the end user
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Conjugate Tag
Alkaline Phosphatase
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Method of Purification
Rabbit anti Human IgG (H + L) (Alk Phos) was purified by affinity chromatography.
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Concentration
1.0 mg/ml
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Form Buffer
0.05M TRIS-HCl, pH 8.0, with 0.15M NaCl, 0.001M MgCl, 0.0001M ZnCl, 50% glycerol, 10 mg/ml BSA, and 0.01% NaN3. I mMunoglobulin and protease free.
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Storage
Store at 4 deg C. DO NOT FREEZE.
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Shipping conditions
Blue Ice
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Tested for
ELISA; IHC; WB
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Usage Recommendations
ELISA: 1:2,000-1:10,000, IHC: 1:200-1:1,000, WB: 1:500-1:2,500
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Short name
Rabbit anti IgG H + L Alk Phos
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Technique
anti human, Rabbit, anti, IgG, antibody to, anti human antibodies, IgGs
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Host
Rabbit, Rabbits
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Isotype
IgG, IgG
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Species
Human, Humans
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Alternative name
Rabbit anti Human IgG (H + L) secondary antibody (Alk Phos)
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Alternative technique
rabbit-anti, antibodies, immunoglobulins
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Alternative to gene target
anaplastic lymphoma receptor tyrosine kinase, CD246 and NBLST3, ALK and IDBG-42084 and ENSG00000171094 and 238, transferase activity, Plasma membranes, Alk and IDBG-197259 and ENSMUSG00000055471 and 11682, ALK and IDBG-636042 and ENSBTAG00000007379 and 536642
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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