E. coli Endonuclease III Enzyme & Buffer

  • Catalog number
    4045-01K-EB
  • Price
    Please ask
  • Size
    1000 units
  • Keep at
    -20° C
  • Assay performance
    The FLARE Sample Prep (slides, lysis solution and low melt agarose) are for use with the FLARE Modules, enzyme and buffers for FLARE, Fragment Length Analysis using Repair Enzymes, detecting DNA damage in single cells using E. coli formamidopyrimidine-DNA glycosylase (Fpg) with single cell gel electrophoresis for DNA damage from mutagens, drugs, Fpg catalyzes the excision of the following forms of DNA damage:opening from 7-methylguanine, including 2,6-diamino-4-hydroxy-5-N-methylformamido-pyrimidine, and 4,6-diamino-5-formamidopyimidine, 8- OHdG, 4-HNA or oxoguaninelesions 5-hydroxycytosine and 5-hydroxyuracil, Aflatoxin-bound imidazole-ring-opened guanine, Imidazole ring opened N-2-aminofluorene-C8-guanine. The FLARE slides are then immersed in an alkali solution, to unwind and denature the DNA strands prior to alkali gel electrophoresis.
  • More advice
    The FLARE Sample Preparation kit contains the necessary comet assay reagents for preparing your samples for incubation with DNA Repair Enzymes to determine the DNA damage occurred by substrate specificity like by Fpg Comet is used to see the tail length, DNA% in the tail, and tail moment. Trevigen FLARE Slides, in FLARE Assay Kits contain low melting agarose and are not used in diagnostic procedures
  • Description
    Buffering solutions are useful to keep the pH range sable when using this reagent of Trevigen. Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
  • Gene target
  • Gene symbol
    CPLX3, MGAT3, MGST3, P4HA3, CASP5, IGHVIII-82, THAP4, MT-CSB3, IGHVIII-44, IGHVIII-67-4
  • Short name
    Endonuclease III Enzyme &
  • Technique
    Enzyme, enzymes
  • Host
    e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. Trevigen supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
  • Species
    E. coli, E. coli
  • Alternative name
    E. coli Endonuclease III Enzyme & solution
  • Alternative technique
    enzymes, escherichia
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
  • Synonyms gene name
    • mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1993-08-27
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Mannosyl-glycoprotein N-acetylglucosaminyltransferases
  • VEGA ID
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    caspase 5
  • Synonyms gene name
    • caspase 5, apoptosis-related cysteine protease
    • caspase 5, apoptosis-related cysteine peptidase
  • Synonyms
  • Locus
  • Discovery year
    1996-09-13
  • Entrez gene record
    838
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Caspases
    • Caspase recruitment domain containing
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable (III)-82 (pseudogene)
  • Synonyms gene name
    • immunoglobulin heavy variable (III)-82
    • immunoglobulin heavy variable (III)-82 pseudogene
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-17
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin heavy locus at 14q32.33
  • VEGA ID
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    mitochondrially encoded conserved sequence block III
  • Synonyms gene
  • Synonyms gene name
    • conserved sequence block III
  • Synonyms
  • Locus
  • Discovery year
    1989-10-11
  • Pubmed identfication
  • Classification
    • Mitochondrially encoded regions
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable (III)-44 (pseudogene)
  • Synonyms gene name
    • immunoglobulin heavy variable (III)-44
    • immunoglobulin heavy variable (III)-44 pseudogene
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-17
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin heavy locus at 14q32.33
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable (III)-67-4 (pseudogene)
  • Synonyms gene name
    • immunoglobulin heavy variable (III)-67-4
    • immunoglobulin heavy variable (III)-67-4 pseudogene
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-17
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin heavy locus at 14q32.33
  • VEGA ID
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