HLA-G Exo-Flow capture kit (Magnetic streptavidin beads, HLA-G-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain)
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Catalog numberEXOFLOW600A-1
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PricePlease ask
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Size10 reactions
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Shipping TemperatureBlue Ice
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Product categoryExosomes
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PropertiesIf you buy Antibodies supplied by Systembio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. This Systembio Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Systembio FITC is highly sensitive to pH extremes.
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ConjugationBiotinylated, Anti-FITC Antibody, Magnetic beads
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TestFlow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.
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French translationanticorps
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Gene target
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Gene symbolHLA-G, IGHV3-71, HLA-DPA2, HLA-DOA, HLA-DQA1, HLA-N, HLA-DQA2, HLA-DPB1, HLA-DQB2, HLA-W
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Short nameHLA-G Exo- capture kit (Magnetic streptavidin , HLA-G- capture antibody, Elution Buffers, Exo-FITC stain)
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TechniqueAntibody, FITC, Flow, antibodies against human proteins, antibodies for, All kind of bead conjugates are available, magnetic, borosilicate, agarose protein A. magnetic beads can be isolated by a macs type magnet. Fluorescein
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LabelMagnetic
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Alternative namemajor histocompatibility complex, class I, G Exo-Flow capture reagent (Magnetic biotinilated-binding streptavidin particles, major histocompatibility complex, class I, G-biotinilated capture (antibody to-), washing and Elution Buffers, Exo-fluorecein staining)
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Alternative techniqueantibodies, fluorescine, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, G
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Synonyms gene name
- HLA-G histocompatibility antigen, class I, G
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Synonyms name
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Locus
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Discovery year1990-09-10
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Entrez gene record
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RefSeq identity
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Classification
- C1-set domain containing
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-71 (pseudogene)
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Synonyms gene
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Synonyms gene name
- immunoglobulin heavy variable 3-71
- immunoglobulin heavy variable 3-G pseudogene (provisional)
- immunoglobulin heavy variable 3-G (provisional)
- immunoglobulin heavy variable 3-71 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class II, DP alpha 2 (pseudogene)
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Synonyms gene
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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Classification
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class II, DO alpha
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C1-set domain containing
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class II, DQ alpha 1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2001-06-22
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Entrez gene record
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RefSeq identity
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Classification
- C1-set domain containing
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, N (pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-10-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class II, DQ alpha 2
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Synonyms gene
-
Locus
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Discovery year2001-06-22
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Entrez gene record
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RefSeq identity
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Classification
- C1-set domain containing
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class II, DP beta 1
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Synonyms gene
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Locus
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Discovery year2001-06-22
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Entrez gene record
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RefSeq identity
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Classification
- C1-set domain containing
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class II, DQ beta 2
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Synonyms gene
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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Classification
- C1-set domain containing
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, W (pseudogene)
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Synonyms gene
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Synonyms gene name
- HLA-80 pseudogene
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GenBank acession
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Locus
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Discovery year2004-01-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data