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Target
VEGFR2 (pTyr1175)
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Conjugate
FITC
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Research Area s
Cardiovascular System, Angiogenesis, Cardiovascular Growth Factors, VEGF, VEGF Receptors, Cell Signaling, Protein Phosphorylation, Tyrosine Kinases, Receptor Tyrosine Kinases, Angiogenesis, Cancer, Tumor Biomarkers, Oncoproteins, Growth Factor Receptors, Stem Cells, Endothelial Progenitors, EP Endothelial Markers, Cancer, Cancer Metabolism, Response to Hypoxia, Metabolism
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Alternative Name s
Protein tyrosine kinase receptor FLK1 Antibody, EC 2.7.10.1 Antibody, VEGFR-2 Antibody, KDR Antibody, Tyrosine kinase growth factor receptor Antibody, VEGFR 2 Antibody, VEGFR Antibody, Fetal liver kinase 1 Antibody, CD309 antigen Antibody, VEGFR2 Antibody, Kinase insert domain receptor (a type III receptor tyrosine kinase) Antibody, KRD1 Antibody, FLK-1 Antibody, FLK1 Antibody, EC:2.7.10.1 Antibody, CD309 Antibody, VGFR2_HUMAN Antibody, Ly73 Antibody, CD_antigen: CD309 Antibody, FLK1, mouse, homolog of Antibody, soluble VEGFR2 Antibody, Vascular endothelial growth factor receptor 2 Antibody, Protein-tyrosine kinase receptor flk-1 Antibody, Kinase insert domain receptor Antibody
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Category
Antibodies
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Product Type
Polyclonal
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Immunogen
Synthetic phospho-peptide from human VEGFR2 around the phosphorylation site of tyrosine 1175 (pTyr1175).
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Immunogen Species
Human
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Accession Number
NP_002244.1
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Gene ID
3791
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Swiss Prot
P35968
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Applications
WB, IHC, ELISA
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Host Species
Rabbit
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Species Reactivity
Human, Mouse, Rat
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Antibody Dilution
WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000); optimal dilutions for assays should be determined by the user.
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Purification
Peptide Affinity Purified
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Storage Buffer
PBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide
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Specificity
Detects endogenous levels of VEGFR2 protein only when phosphorylated at tyrosine 1175 (pTyr1175). Detects a band at ~152 kDa.
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Storage Temperature
-20ºC
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Shipping Temperature
Blue Ice or 4ºC
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Cellular Localization
Endoplasmic Reticulum, Cell Junction, Plasma Membrane, Cell Membrane, Single-Pass Type I Membrane Protein, Nucleus, Endosome, Early Endosome, Cytoplasm, Cytoplasmic Vesicle, Secreted, Sorting Endosome, Extracellular Region, Golgi Apparatus, Integral Component Of Plasma Membrane, Membrane Raft
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Field of Use
For in vitro research use only.
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Gross Weight kg
1.4
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Net Weight g
0.1
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Properties
If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This StressMarq Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMarq FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
