STAT5A Antibody (pSer780): FITC
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Catalog number
SPC-1432D-FITC
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Price
Please ask
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Size
100 µl
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Target
STAT5A (pSer780)
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Conjugate
FITC
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Research Area s
Cell Signaling, Epigenetics and Nuclear Signaling, Nuclear Signaling Pathways, STATs
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Alternative Name s
Signal transducer and activator of transcription 5A Antibody, STAT5A Antibody, STAT5 Antibody
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Category
Antibodies
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Product Type
Polyclonal
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Immunogen
Synthetic phospho-peptide from human STAT5A around the phosphorylation site of serine 780 (pSer780).
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Immunogen Species
Human
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Accession Number
NP_001275647.1
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Gene ID
6776
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Swiss Prot
P42229
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Applications
WB, IHC, IP, ELISA
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Host Species
Rabbit
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Species Reactivity
Human, Mouse, Rat
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Antibody Dilution
WB (1:250-1:1000); IHC (1:50-1:150); IP (1-2.5 ug per mg lysate); ELISA (1:5000); optimal dilutions for assays should be determined by the user.
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Purification
Peptide Affinity Purified
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Storage Buffer
PBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide
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Specificity
Detects endogenous levels of STAT5A protein only when phosphorylated at serine 780 (pSer780). Detects a band at ~91 kDa.
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Storage Temperature
-20ºC
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Shipping Temperature
Blue Ice or 4ºC
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Cellular Localization
Nucleus, Cytoplasm, Cytosol, Nucleoplasm
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Field of Use
For in vitro research use only.
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Gross Weight kg
1.4
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Net Weight g
0.1
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Properties
If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This StressMarq Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMarq FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
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Gene target
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Gene symbol
STAT5A
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Short name
STAT5A Antibody (pSer780): FITC
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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Label
FITC
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Alternative name
signal transducer and activator on transcription 5A (antibody to-) (pSer780): fluorecein
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Alternative technique
antibodies, fluorescine
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Alternative to gene target
signal transducer and activator of transcription 5A, MGF and STAT5, STAT5A and IDBG-50625 and ENSG00000126561 and 6776, protein phosphatase binding, nuclei, Stat5a and IDBG-211618 and ENSMUSG00000004043 and 20850, STAT5A and IDBG-640187 and ENSBTAG00000009496 and 282375
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Gene info
MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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