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Target
Smad3 (pSer425)
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Conjugate
PerCP
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Research Area s
Cell Signaling, Epigenetics and Nuclear Signaling, Nuclear Signaling Pathways, SMADs, Stem Cells, Signaling Pathways, TGF Beta, Cytoplasmic, Cancer, Cancer Metabolism, Response to Hypoxia, Cell Signaling, Metabolism, Metabolism processes, Hypoxia
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Alternative Name s
Smad3 Antibody, Mothers against decapentaplegic homolog 3 Antibody, Mad3 Antibody, SMAD3_HUMAN Antibody, DKFZp686J10186 Antibody, SMAD Antibody, HSPC193 Antibody, SMAD family member 3 Antibody, hMAD-3 Antibody, MGC60396 Antibody, MAD homolog 3 Antibody, HST17436 Antibody, JV15-2 Antibody, MAD (mothers against decapentaplegic Drosophila) homolog 3 Antibody, JV152 Antibody, Mad homolog JV15 2 Antibody, SMAD 3 Antibody, JV15 2 Antibody, DKFZP586N0721 Antibody, MADH3 Antibody, MAD, Mothers against DPP homolog 3 Antibody, hSMAD3 Antibody, MADH 3 Antibody, LDS3 Antibody, Mad protein homolog Antibody, SMA and MAD related protein 3 Antibody, LDS1C Antibody, hMAD 3 Antibody
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Category
Antibodies
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Product Type
Polyclonal
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Immunogen
Synthetic phospho-peptide from human Smad3 around the phosphorylation site of serine 425 (pSer425).
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Immunogen Species
Human
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Accession Number
NP_001138574.1
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Gene ID
4088
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Swiss Prot
P84022
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Applications
WB, IHC
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Host Species
Rabbit
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Species Reactivity
Human, Mouse, Rat
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Antibody Dilution
WB (1:250-1:1000); IHC (1:25-1:100); optimal dilutions for assays should be determined by the user.
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Purification
Peptide Affinity Purified
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Storage Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide
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Specificity
Detects endogenous levels of Smad3 only when phosphorylated at serine 425 (pSer425). Detects a band at ~58 kDa.
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Storage Temperature
-20ºC
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Shipping Temperature
Blue Ice or 4ºC
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Cellular Localization
Nucleus, Cytoplasm, Cytosol, Nuclear Chromatin, Nuclear Inner Membrane, Nucleoplasm, Plasma Membrane, Receptor Complex
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Field of Use
For in vitro research use only.
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Gross Weight kg
1.4
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Net Weight g
0.1
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Properties
If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Additional conjugate
Peridinin chlorophyll (PerCP)
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French translation
anticorps
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Gene target
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Short name
Smad3 Antibody (pSer425): PerCP
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Technique
Antibody, Percp, antibodies against human proteins, antibodies for, Peridinin chlorophyll protein (PerCP) has a 677 nanometer maximum emission, red, when excited at 488 nanometers and the Smad3 Antibody (pSer425): PerCP is detected on the FL3 detector of a FACSCalibur or FACScan. A PerCP dye is also available (PerCP-Cy5.5, also written Cy5.5-PerCP). PerCP is not suited for the high-powered lasing (>150mW) applications, such as on a MoFlo, due to it photobleaching (features/ qualities/ traits). PerCP conjugates can only be received/be gotten from the Becton Dickinson/Pharmingen group of companies or from StressMarq.
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Isotype
IgG
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Label
PerCP
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Alternative name
SMAD family member 3 (antibody to-) (pSer425): PerCP
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Alternative technique
antibodies, antibodies-percp
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Alternative to gene target
SMAD family member 3, HSPC193 and HsT17436 and JV15-2 and LDS1C and LDS3 and MADH3, SMAD3 and IDBG-18366 and ENSG00000166949 and 4088, transforming growth factor beta receptor, nuclei, Smad3 and IDBG-175693 and ENSMUSG00000032402 and 17127, SMAD3 and IDBG-645466 and ENSBTAG00000012599 and 515125
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