PTEN Antibody (pSer370): FITC

• Catalog number
  SPC-1423D-FITC
• Price
  Please ask
• Size
  100 µl

• Target
  PTEN (pSer370)
• Conjugate
  FITC
• Research Area s
  Cancer, Cell Cycle, Cell Signaling, Lipid Signaling, Lipid Phosphatases, Cell Signaling, Epigenetics and Nuclear Signaling, Transcription, Cancer, Tumor suppressors, PTEN Pathway, Cancer, Tumor Biomarkers, Oncoproteins, Cell Signaling, Metabolism, Diabetes, Cell Signaling, Protein Phosphorylation, Other Phosphatases
• Alternative Name s
  PTEN_HUMAN Antibody, MMAC1 phosphatase and tensin homolog deleted on chromosome 10 Antibody, DEC Antibody, 10q23del Antibody, EC:3.1.3.48, Mutated in multiple advanced cancers 1 Antibody, GLM2 Antibody, MGC11227 Antibody, PTEN Antibody, PTEN1 Antibody, MMAC1 Antibody, 5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN Antibody, Phosphatase and tensin like protein Antibody, Antibody, Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN Antibody, TEP1 Antibody, BZS Antibody, EC:3.1.3.16, Phosphatase and tensin homolog Antibody, 4, Phosphatidylinositol 3, MHAM Antibody, EC:3.1.3.67 Antibody
• Category
  Antibodies
• Product Type
  Polyclonal
• Immunogen
  Synthetic phospho-peptide from human PTEN around the phosphorylation site of serine 370 (pSer370).
• Immunogen Species
  Human
• Accession Number
  NP_000305.3
• Gene ID
  5728
• Swiss Prot
  P60484
• Applications
  WB, IHC, ELISA
• Host Species
  Rabbit
• Species Reactivity
  Human, Mouse, Rat
• Antibody Dilution
  WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:2500); optimal dilutions for assays should be determined by the user.
• Purification
  Peptide Affinity Purified
• Storage Buffer
  PBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide
• Specificity
  Detects endogenous levels of PTEN protein only when phosphorylated at serine 370 (pSer370). Detects a band at ~47 kDa.
• Storage Temperature
  -20ºC
• Shipping Temperature
  Blue Ice or 4ºC
• Cellular Localization
  Nucleus Pml Body, Nucleus, Pml Body, Cytoplasm, Secreted, Cytosol, Extracellular Region, Mitochondrion, Nucleoplasm, Plasma Membrane, Apical Plasma Membrane_x000D_
• Field of Use
  For in vitro research use only.
• Technical data
• SDS
• Gross Weight kg
  1.4
• Net Weight g
  0.1
• Properties
  If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This StressMarq Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMarq FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  PTEN   pSer370  
• Gene symbol
  PTEN, PTENP1
• Short name
  PTEN Antibody (pSer370): FITC
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
• Label
  FITC
• Alternative name
  phosphatase and tensin homolog (antibody to-) (pSer370): fluorecein
• Alternative technique
  antibodies, fluorescine
• Alternative to gene target
  phosphatase and tensin homolog, 10q23del and BZS and CWS1 and DEC and GLM2 and MHAM and MMAC1 and PTEN1 and TEP1, PTEN and IDBG-81409 and ENSG00000171862 and 5728, phosphatidylinositol-3, nuclei, Pten and IDBG-158878 and ENSMUSG00000013663 and 19211, PTEN and IDBG-637256 and ENSBTAG00000009498 and 540786

Gene info

• Identity
  HGNC:9588
• Gene
  PTEN
• Long gene name
  phosphatase and tensin homolog
• Synonyms gene
  • BZS
  • MHAM
• Synonyms
  • MMAC1
  • TEP1
  • PTEN1
• Synonyms name
  • mutated in multiple advanced cancers 1
• GenBank acession
  • U92436
• Locus
  10q23.31
• Discovery year
  1997-04-21
• Entrez gene record
  5728
• Pubmed identfication
  • 9090379
• RefSeq identity
  • NM_000314
• Classification
  • PTEN protein phosphatases
  • C2 tensin-type domain containing
  • Phosphoinositide phosphatases
• VEGA ID
  OTTHUMG00000018688
• Locus Specific Databases
  • LRG_311

Gene info

• Identity
  HGNC:9589
• Gene
  PTENP1
• Long gene name
  phosphatase and tensin homolog pseudogene 1
• Synonyms gene name
  • phosphatase and tensin homolog pseudogene 1 (functional)
• Synonyms
  • PTEN2
  • psiPTEN
  • PTH2
  • PTEN-rs
  • PTENpg1
• GenBank acession
  • AF023139
  • BC038293
  • BY797336
• Locus
  9p13.3
• Discovery year
  1999-09-09
• Entrez gene record
  11191
• Pubmed identfication
  • 9393738
  • 9620558
  • 20577206
• RefSeq identity
  • NR_023917
• VEGA ID
  OTTHUMG00000000410

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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