p53 Antibody (pSer315): Biotin

• Catalog number
  SPC-1411D-BI
• Price
  Please ask
• Size
  100 µl

• Target
  p53 (pSer315)
• Conjugate
  Biotin
• Research Area s
  Cancer, Cell Cycle, p53 Pathway, Cancer, Tumor suppressors, Apoptosis, Cell Signaling, Epigenetics and Nuclear Signaling, DNA/RNA, DNA Damage and Repair
• Alternative Name s
  p53 tumor suppressor Antibody, Cellular tumor antigen p53 Antibody, Antigen NY-CO-13 Antibody, Phosphoprotein p53 Antibody, Tumor protein 53 Antibody, BCC7 Antibody, p53 Antibody, TP53 Antibody, P53_HUMAN Antibody, FLJ92943 Antibody, Mutant tumor protein 53 Antibody, Tumor protein p53 Antibody, LFS1 Antibody, Transformation related protein 53 Antibody, TRP53 Antibody, Tumor suppressor p53 Antibody
• Category
  Antibodies
• Product Type
  Polyclonal
• Immunogen
  Synthetic phospho-peptide from human p53 around the phosphorylation site of serine 315 (pSer315).
• Immunogen Species
  Human
• Accession Number
  NP_000537.3
• Gene ID
  7157
• Swiss Prot
  P04637
• Applications
  WB, IHC, IP, ELISA
• Host Species
  Rabbit
• Species Reactivity
  Human
• Antibody Dilution
  WB (1:250-1:1000); IHC (1:50-1:150); IP (1-2.5 ug per mg lysate); ELISA (1:5000); optimal dilutions for assays should be determined by the user.
• Purification
  Peptide Affinity Purified
• Storage Buffer
  PBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide
• Specificity
  Detects endogenous levels of p53 protein only when phosphorylated at serine 315 (pSer315). Detects a band at ~43 kDa.
• Storage Temperature
  -20ºC
• Shipping Temperature
  Blue Ice or 4ºC
• Cellular Localization
  Mitochondrion Matrix, Endoplasmic Reticulum, Nucleus Pml Body, Nucleus, Pml Body, Cytoplasm, Cytosol, Mitochondrion, Nuclear Chromatin, Nuclear Matrix, Nucleolus, Nucleoplasm, Intracellular, Protein Complex, Replication Fork
• Field of Use
  For in vitro research use only.
• Technical data
• SDS
• Gross Weight kg
  1.4
• Net Weight g
  0.1
• Properties
  If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• French translation
  anticorps

• Gene target
  p53   pSer315  
• Short name
  p53 Antibody (pSer315):
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Label
  Biotin
• Alternative name
  p53 (antibody to-) (pSer315): biotinilated
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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