p27 Kip1 Antibody (pSer10): Biotin
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Catalog numberSPC-1409D-BI
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PricePlease ask
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Size100 µl
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Targetp27 Kip1 (pSer10)
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ConjugateBiotin
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Research Area sCancer, Cell Cycle, Cip/Kip
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Alternative Name sp27 Kip1 Antibody, p27 antiobdy, p27KIP1 Antibody, p27 KIP 1 Antibody, Cyclin-dependent kinase inhibitor 1B Antibody, AA408329 Antibody, AI843786 Antibody, OTTHUMP00000195099 Antibody, MEN4 Antibody, Cyclin dependent kinase inhibitor p27 Antibody, Cyclin Dependent Kinase Inhibitor 1B Antibody, OTTHUMP00000195098 Antibody, CDKN1B Antibody, p27Kip1 Antibody, Cdki1b Antibody, p27 Kip1 Antibody, MEN1B Antibody, CDKN4 Antibody, Cyclin-dependent kinase inhibitor 1B (p27, Kip1) Antibody, P27-like cyclin-dependent kinase inhibitor Antibody, p27 Antibody, CDKN 1B Antibody, Cyclin-dependent kinase inhibitor p27 Kip1 Antibody, CDN1B_HUMAN Antibody, KIP 1 Antibody, Cyclin-dependent kinase inhibitor p27 Antibody, KIP1 Antibody, CDKN 4 Antibody
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CategoryAntibodies
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Product TypePolyclonal
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ImmunogenSynthetic phospho-peptide from human p27 Kip1 around the phosphorylation site of serine 10 (pSer10).
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Immunogen SpeciesHuman
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Accession NumberNP_004055.1
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Gene ID1027
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Swiss ProtP46527
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ApplicationsWB
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Host SpeciesRabbit
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Species ReactivityHuman, Mouse, Rat
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Antibody DilutionWB (1:250-1:1000); optimal dilutions for assays should be determined by the user.
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PurificationPeptide Affinity Purified
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Storage BufferPBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide
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SpecificityDetects endogenous levels of p27 Kip1 only when phosphorylated at serine 10 (pSer10). Detects a band at ~27 kDa.
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Storage Temperature-20ºC
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Shipping TemperatureBlue Ice or 4ºC
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Cellular LocalizationNucleus, Endosome, Cytoplasm, Cytosol, Nucleoplasm, CUL4A-Ring E3 Ubiquitin Ligase Complex, Intracellular Membrane-Bounded Organelle, Protein Complex
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Field of UseFor in vitro research use only.
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Gross Weight kg1.4
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Net Weight g0.1
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PropertiesIf you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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ConjugationBiotinylated
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French translationanticorps
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Gene target
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Short namep27 Kip1 Antibody (pSer10):
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeIgG
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LabelBiotin
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Alternative namep27 Kip1 (antibody to-) (pSer10): biotinilated
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Alternative techniqueantibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data