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Target
mTOR (pSer2448)
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Conjugate
FITC
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Research Area s
Cancer, Cell Cycle, Cell Cycle Inhibitors, Cell Signaling, Epigenetics and Nuclear Signaling, DNA/RNA, DNA Damage and Repair, DNA Damage Recognition, Cardiovascular System, Heart, Cardiogenesis, Transcription Factors / Regulators
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Alternative Name s
FK506 binding protein 12 rapamycin associated protein 2 Antibody, FLJ44809 Antibody, FRAP1 Antibody, Rapamycin target protein Antibody, FRAP Antibody, Rapamycin target protein 1 Antibody, OTTHUMP00000001983 Antibody, EC:2.7.11.1 Antibody, FK506-binding protein 12-rapamycin complex-associated protein 1 Antibody, FKBP12-rapamycin complex-associated protein Antibody, MTOR Antibody, RAPT1 Antibody, Mammalian target of rapamycin Antibody, Serine/threonine-protein kinase mTOR Antibody, MTOR_HUMAN Antibody, FK506 binding protein 12 rapamycin complex associated protein 1 Antibody, FKBP12-rapamycin complex-associated protein 1 Antibody, dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) Antibody, RAFT1 Antibody, FRAP2 Antibody, FKBP rapamycin associated protein Antibody, FKBP12 rapamycin complex associated protein Antibody, Mechanistic target of rapamycin Antibody, FK506 binding protein 12 rapamycin associated protein 1 Antibody, Rapamycin and FKBP12 target 1 Antibody, Rapamycin associated protein FRAP2 Antibody
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Category
Antibodies
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Product Type
Polyclonal
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Immunogen
Synthetic phospho-peptide from human mTOR around the phosphorylation site of serine 2448 (pSer2448).
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Immunogen Species
Human
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Accession Number
NP_004949.1
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Gene ID
2475
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Swiss Prot
P42345
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Applications
WB, IHC
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Host Species
Rabbit
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Species Reactivity
Human, Mouse, Rat
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Antibody Dilution
WB (1:250-1:1000); IHC (1:25-1:100); optimal dilutions for assays should be determined by the user.
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Purification
Peptide Affinity Purified
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Storage Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide
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Specificity
Detects endogenous levels of mTOR only when phosphorylated at serine 2448 (pSer2448). Detects a band at ~289 kDa.
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Storage Temperature
-20ºC
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Shipping Temperature
Blue Ice or 4ºC
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Cellular Localization
Mitochondrion, Mitochondrion Outer Membrane, Peripheral Membrane Protein, Cytoplasmic Side, Endoplasmic Reticulum Membrane, Microsome Membrane, Nucleus, PML Body, Lysosome, Golgi Apparatus Membrane, Cytoplasm, Cytosol, Golgi Apparatus, Golgi Membrane, Lysosomal Membrane, Nucleoplasm, Dendrite, Endomembrane System, Membrane, Neuronal Cell Body, Phosphatidylinositol 3-Kinase Complex, Torc1 Complex, Torc2 Complex
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Field of Use
For in vitro research use only.
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Gross Weight kg
1.4
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Net Weight g
0.1
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Properties
If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This StressMarq Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMarq FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
