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Target
HER2 (pTyr877)
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Conjugate
FITC
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Research Area s
Cell Signaling, Protein Phosphorylation, Tyrosine Kinases, Receptor Tyrosine Kinases, Cell Signaling, Growth Factors, EGF, Cancer, Tumor Biomarkers, Oncoproteins
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Alternative Name s
HER2 Antibody, ERBB2 Antibody, Neu Antibody, ERbB2 Antibody, C erb B2/neu protein Antibody, Neuroblastoma/glioblastoma derived oncogene homolog Antibody, Tyrosine kinase-type cell surface receptor HER2 Antibody, Tyrosine kinase type cell surface receptor HER2 Antibody, Receptor tyrosine-protein kinase erbB-2 Antibody, V erb b2 avian erythroblastic leukemia viral oncoprotein 2 Antibody, Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog Antibody, HER 2/NEU Antibody, MLN 19 Antibody, V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) Antibody, Human epidermal growth factor receptor 2 Antibody, NGL Antibody, NEU Antibody, CD340 Antibody, CD340 antigen Antibody, Proto-oncogene Neu Antibody, HER 2 Antibody, ERBB2 Antibody, EC:2.7.10.1 Antibody, HER2 Antibody, CerbB2 Antibody, neuro/glioblastoma derived oncogene homolog (avian) Antibody, Herstatin Antibody, V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 Antibody, CD_antigen: CD340 Antibody, TKR1 Antibody, V erb b2 erythroblastic leukemia viral oncogene homolog 2, Cerb B2/neu protein Antibody, Erb b2 receptor tyrosine kinase 2 Antibody, ERBB2_HUMAN Antibody, NEU proto oncogene Antibody, Proto-oncogene c-ErbB-2 Antibody, Metastatic lymph node gene 19 protein Antibody, MLN19 Antibody, p185erbB2 Antibody
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Category
Antibodies
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Product Type
Polyclonal
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Immunogen
Synthetic phospho-peptide from human HER2 around the phosphorylation site of tyrosine 877 (pTyr877).
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Immunogen Species
Human
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Accession Number
NP_001005862.1
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Gene ID
2064
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Swiss Prot
P04626
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Applications
WB, IHC, IP, ELISA
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Host Species
Rabbit
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Species Reactivity
Human, Mouse, Rat
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Antibody Dilution
WB (1:250-1:1000); IHC (1:50-1:150); IP (1-2.5 ug per mg lysate); ELISA (1:5000); optimal dilutions for assays should be determined by the user.
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Purification
Peptide Affinity Purified
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Storage Buffer
PBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide
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Specificity
Detects endogenous levels of HER2 protein only when phosphorylated at tyrosine 877 (pTyr877). Detects a band at ~185 kDa.
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Storage Temperature
-20ºC
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Shipping Temperature
Blue Ice or 4ºC
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Cellular Localization
Plasma Membrane, Cell Membrane, Single-Pass Type I Membrane Protein, Nucleus, Perinuclear Region, Cytoplasm, Cytosol, Endosome, Endosome Membrane, Apical Plasma Membrane, Basolateral Plasma Membrane, Integral Component Of Membrane, Myelin Sheath, Perinuclear Region Of Cytoplasm receptor Complex, Perinuclear Region Of Cytoplasm, Receptor Complex
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Field of Use
For in vitro research use only.
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Gross Weight kg
1.4
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Net Weight g
0.1
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Properties
If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This StressMarq Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMarq FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
