HDAC5 Antibody (pSer498): FITC
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Catalog numberSPC-1387D-FITC
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PricePlease ask
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Size100 µl
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TargetHDAC5 (pSer498)
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ConjugateFITC
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Research Area sCell Signaling, Epigenetics and Nuclear Signaling, Chromatin Modifying Enzymes, DNA Acetylation, HDACS, Stem Cells, Stem Cell HDACs, Cardiovascular System, Heart, Hypertrophy
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Alternative Name sHistone deacetylase 5 Antibody, HDAC5 Antibody, NY CO 9 Antibody, EC:3.5.1.98 Antibody, Antigen NY CO 9 Antibody, HDAC5_HUMAN Antibody, Antigen NY-CO-9 Antibody, HDAC 5 Antibody, KIAA0600 Antibody, HD5 Antibody
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CategoryAntibodies
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Product TypePolyclonal
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ImmunogenSynthetic phospho-peptide from human HDAC5 around the phosphorylation site of serine 498 (pSer498).
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Immunogen SpeciesHuman
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Accession NumberNP_001015053.1
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Gene ID10014
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Swiss ProtQ9UQL6
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ApplicationsWB, IHC, ICC/IF, ELISA
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Host SpeciesRabbit
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Species ReactivityHuman, Mouse
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Antibody DilutionWB (1:250-1:1000); IHC (1:50-1:150); ICC/IF (1:100-1:500); ELISA (1:10000); optimal dilutions for assays should be determined by the user.
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PurificationPeptide Affinity Purified
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Storage BufferPBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide
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SpecificityDetects endogenous levels of HDAC5 protein only when phosphorylated at serine 498 (pSer498). Detects a band at ~122 kDa.
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Storage Temperature-20ºC
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Shipping TemperatureBlue Ice or 4ºC
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Cellular LocalizationNucleus, Cytoplasm, Cytosol, Golgi Apparatus, Histone Deacetylase Complex, Nuclear Speck, Nucleoplasm, Protein Complex
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Field of UseFor in vitro research use only.
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Gross Weight kg1.4
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Net Weight g0.1
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PropertiesIf you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This StressMarq Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMarq FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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French translationanticorps
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Gene target
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Gene symbolHDAC5
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Short nameHDAC5 Antibody (pSer498): FITC
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TechniqueAntibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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LabelFITC
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Alternative namehistone deacetylase 5 (antibody to-) (pSer498): fluorecein
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Alternative techniqueantibodies, fluorescine
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Alternative to gene targethistone deacetylase 5, HD5 and NY-CO-9, HDAC5 and IDBG-53295 and ENSG00000108840 and 10014, NAD-dependent histone deacetylase activity (H3-K18 specific), nuclei, Hdac5 and IDBG-212520 and ENSMUSG00000008855 and 15184, HDAC5 and IDBG-640847 and ENSBTAG00000016254 and 504242
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Gene info
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Identity
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Gene
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Long gene namehistone deacetylase 5
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-11-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Histone deacetylases, class IIA
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data