HDAC4 Antibody (pSer632): FITC

• Catalog number
  SPC-1386D-FITC
• Price
  Please ask
• Size
  100 µl

• Target
  HDAC4 (pSer632)
• Conjugate
  FITC
• Research Area s
  Cell Signaling, Epigenetics and Nuclear Signaling, Chromatin Modifying Enzymes, DNA Acetylation, HDACS, Stem Cells, Stem Cell HDACs, Cardiovascular System, Heart, Hypertrophy
• Alternative Name s
  Histone deacetylase 4 Antibody, BDMR Antibody, HDAC4 Antibody, HDACA Antibody, EC:3.5.1.98 Antibody, HDAC 4 Antibody, KIAA0288 Antibody, AHO3 Antibody, HA6116 Antibody, HDAC A Antibody, Histone Deacetylase A Antibody, HD4 Antibody, HD 4 Antibody, HDAC4_HUMAN Antibody, EC 3.5.1.98 Antibody
• Category
  Antibodies
• Product Type
  Polyclonal
• Immunogen
  Synthetic phospho-peptide from human HDAC4 around the phosphorylation site of serine 632 (pSer632).
• Immunogen Species
  Human
• Accession Number
  NP_006028.2
• Gene ID
  9759
• Swiss Prot
  P56524
• Applications
  WB, ELISA
• Host Species
  Rabbit
• Species Reactivity
  Human, Mouse, Rat
• Antibody Dilution
  WB (1:250-1:500); ELISA (1:10000); optimal dilutions for assays should be determined by the user.
• Purification
  Peptide Affinity Purified
• Storage Buffer
  PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide
• Specificity
  Detects endogenous levels of HDAC4 only when phosphorylated at serine 632 (pSer632).
• Storage Temperature
  -20ºC
• Shipping Temperature
  Blue Ice or 4ºC
• Certificate of Analysis
  A 1:500 dilution of SPC-1386 was sufficient for detection of HDAC4 (pSer632) in 10 µg of human jurkat cell lysates by ECL immunoblot analysis using goat anti-rabbit as the secondary antibody.
• Cellular Localization
  Nucleus, Cytoplasm, Cytoskeleton, Actomyosin, Cytosol, Histone Deacetylase Complex, Nucleoplasm, Neuromuscular Junction, Protein Complex, Transcriptional Repressor Complex, Z Disc, A Band
• Field of Use
  For in vitro research use only.
• Technical data
• SDS
• Gross Weight kg
  1.4
• Net Weight g
  0.1
• Properties
  If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This StressMarq Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMarq FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  HDAC4   pSer632  
• Gene symbol
  HDAC4-AS1, HDAC4
• Short name
  HDAC4 Antibody (pSer632): FITC
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
• Isotype
  IgG
• Label
  FITC
• Alternative name
  histone deacetylase 4 (antibody to-) (pSer632): fluorecein
• Alternative technique
  antibodies, fluorescine
• Alternative to gene target
  histone deacetylase 4, AHO3 and BDMR and HA6116 and HD4 and HDAC-4 and HDAC-A and HDACA, HDAC4 and IDBG-84594 and ENSG00000068024 and 9759, NAD-dependent histone deacetylase activity (H3-K18 specific), nuclei, Hdac4 and IDBG-181579 and ENSMUSG00000026313 and 208727, HDAC4 and IDBG-642244 and ENSBTAG00000017764 and 517559

Gene info

• Identity
  HGNC:54064
• Gene
  HDAC4-AS1
• Long gene name
  HDAC4 antisense RNA 1
• Locus
  2q37.3
• Discovery year
  2018-09-27
• Entrez gene record
  101928111
• RefSeq identity
  • NR_135575
• Classification
  • Antisense RNAs

Gene info

• Identity
  HGNC:14063
• Gene
  HDAC4
• Long gene name
  histone deacetylase 4
• Synonyms gene
  • BDMR
• Synonyms gene name
  • brachydactyly-mental retardation syndrome
• Synonyms
  • KIAA0288
  • HDAC-A
  • HDACA
  • HD4
  • HA6116
  • HDAC-4
• GenBank acession
  • AB006626
• Locus
  2q37.3
• Discovery year
  2000-11-28
• Entrez gene record
  9759
• Pubmed identfication
  • 10206986
  • 10220385
  • 20691407
• RefSeq identity
  • NM_006037
• Classification
  • Histone deacetylases, class IIA
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000133344

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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