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Target
Fyn (pTyr530)
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Conjugate
FITC
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Research Area s
Neuroscience, Neurodegeneration, Alzheimer's Disease, Cell Signaling, Protein Phosphorylation, Tyrosine Kinases, Src Family, Cancer, Tumor Biomarkers, Oncoproteins, Signal Transducers
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Alternative Name s
Proto oncogene tyrosine protein kinase fyn Antibody, Proto-oncogene c-Fyn Antibody, SLK Antibody, FYN oncogene related to SRC FGR YES Antibody, Src yes related novel gene Antibody, Protein tyrosine kinase fyn Antibody, Src like kinase Antibody, Fyn Antibody, OKT3 induced calcium influx regulator Antibody, Protooncogene Syn Antibody, Tyrosine kinase p59fyn T Antibody, SYN Antibody, EC:2.7.10.2 Antibody, Tyrosine-protein kinase Fyn Antibody, FYN_HUMAN Antibody, C syn protooncogene Antibody, Proto-oncogene Syn Antibody, Tyrosine kinase p59fyn(T) Antibody, p59-Fyn Antibody, Src/yes related novel Antibody, Src-like kinase Antibody, P59 FYN Antibody
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Category
Antibodies
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Product Type
Polyclonal
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Immunogen
Synthetic phospho-peptide from human Fyn around the phosphorylation site of tyrosine 530 (pTyr530).
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Immunogen Species
Human
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Accession Number
NP_002028.1
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Gene ID
2534
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Swiss Prot
P06241
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Applications
WB, IHC, ELISA
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Host Species
Rabbit
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Species Reactivity
Human, Mouse
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Antibody Dilution
WB (1:250-1:500); IHC (1:25-1:50); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
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Purification
Peptide Affinity Purified
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Storage Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide
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Specificity
Detects endogenous levels of Fyn only when phosphorylated at tyrosine 530 (pTyr530).
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Storage Temperature
-20ºC
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Shipping Temperature
Blue Ice or 4ºC
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Certificate of Analysis
A 1:500 dilution of SPC-1383 was sufficient for detection of Fyn (pTyr530) in 10 µg of human embryonic kidney epithelial cell line (hek293) lysate by ECL immunoblot analysis using goat anti-rabbit as the secondary antibody.
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Cellular Localization
Plasma Membrane, Cell Membrane, Nucleus, Cytoplasm, Cytoskeleton, Actin Filament, Cytosol, Endosome, Mitochondrion, Extrinsic Component Of Cytoplasmic Side Of Plasma Membrane, Dendrite, Membrane Raft
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Field of Use
For in vitro research use only.
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Gross Weight kg
1.4
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Net Weight g
0.1
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Properties
If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This StressMarq Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMarq FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
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Gene target
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Short name
Fyn Antibody (pTyr530): FITC
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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Isotype
IgG
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Label
FITC
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Alternative name
FYN oncogene related to v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian), FGR, YES (antibody to-) (pTyr530): fluorecein
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Alternative technique
antibodies, fluorescine
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Alternative to gene target
FYN oncogene related to SRC, FGR, YES, p59-FYN and SLK and SYN, FYN and IDBG-95463 and ENSG00000010810 and 102724705,2534, transferase activity, nuclei, Fyn and IDBG-144629 and ENSMUSG00000019843 and 14360, FYN and IDBG-631420 and ENSBTAG00000011851 and 527263
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